摘要
目的
观察经皮穴位电刺激(Transcutaneous Electrical Acupoint Stimulation, TEAS)对运动性疲劳大鼠的影响。
方法
采用递增负荷跑台法建立大鼠慢性运动性疲劳模型, 第6 星期开始, 分别在运动后即刻和3 h 后进行TEAS治疗。治疗2 星期后, HE 染色观察大鼠海马、下丘脑组织形态学变化, 免疫组化法观察海马、下丘脑5 — 羟色胺(5-hydroxytryptamine, 5-HT)的表达。
结果
TEAS 可以明显减轻运动引起的大鼠海马、下丘脑组织形态损伤, 降低大鼠海马、下丘脑5-HT 表达, 运动后即刻治疗和3 h 后治疗的两组间无统计学差异。
结论
TEAS 有促进疲劳恢复, 提高运动能力的作用。TEAS 改善大鼠的运动性疲劳状态与降低中枢5-HT 表达、减少神经细胞损伤有关。
Abstract
Objective
To observe the effect of transcutaneous electrical acupoint stimulation (TEAS) on the rats with exercise-induced fatigue.
Methods
Rat model of chromic exercise-induced fatigue was established with increasing load treadmill walking method. The TEAS therapy was operated immediately and 3 h after the exercise from the sixth week. Hippocampal morphology and hypothalamic morphology were observed with HE staining and the expressions of 5-hydroxytryptamine (5-HT) in hippocampus and hypothalamus were tested with immunohistochemical (IHC) staining after 2-week treatment.
Results
TEAS released the damage of hippocampal morphology and hypothalamic morphology induced by exercise significantly and reduced the expression of 5-HT in hippocampus and hypothalamus. The difference between the therapy operated immediately and 3 h after exercise was not significant.
Conclusion
TEAS could improve the fatigue recovery and athletic ability. The improvement of TEAS on the rats with exercise-induced fatigue was related with the decreased expression of central 5-HT and the reduction of damaged nerve cells.
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Fang, Jq., Ma, Gz. & Liang, Y. Effect of transcutaneous electrical acupoint stimulation on rats with chronic exercise-induced fatigue. J. Acupunct. Tuina. Sci. 10, 265–270 (2012). https://doi.org/10.1007/s11726-012-0617-6
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DOI: https://doi.org/10.1007/s11726-012-0617-6