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Sodium carboxymethylation-functionalized chitosan fibers for cutaneous wound healing application

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Abstract

A water absorption biomaterial, sodium carboxymethylation-functionalized chitosan fibers (Na-NOCC fibers) were prepared, applied for cutaneous wound repair, and characterized by FTIR and NMR. The water absorption of Na-NOCC fibers increased significantly with substitution degree rising, from 3.2 to 6.8 g/g, and higher than that of chitosan fibers (2.2 g/g) confirmed by swelling behavior. In the antibacterial action, the high degree of substitution of Na-NOCC fibers exhibited stronger antibacterial activities against E. coli (from 66.54% up to 88.86%). The inhibition of Na-NOCC fibers against S. aureus were above 90%, and more effective than E. coli. The cytotoxicity assay demonstrated that Na-NOCC2 fibers were no obvious cytotoxicity to mouse fibroblasts. Wound healing test and histological examination showed that significantly advanced granulation tissue and capillary formation in the healing-impaired wounds treated with Na-NOCC fibers, as compared to those treated with gauze, which demonstrated that Na- NOCC fibers could promote skin repair and might have great application for wound healing.

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References

  1. Miraftab M, Masood R, Edward-Jones V. A new carbohydratebased wound dressing fibre with superior absorption and antimicrobial potency. Carbohydrate Polymers, 2014, 101: 1184–1190

    Article  Google Scholar 

  2. Babu R, Zhang J, Beckman E J, et al. Antimicrobial activities of silver used as a polymerization catalyst for a wound-healing matrix. Biomaterials, 2006, 27(24): 4304–4314

    Article  Google Scholar 

  3. Kanokpanont S, Damrongsakkul S, Ratanavaraporn J, et al. An innovative bi-layered wound dressing made of silk and gelatin for accelerated wound healing. International Journal of Pharmaceutics, 2012, 436(1–2): 141–153

    Article  Google Scholar 

  4. Lu S, Gao W, Gu H Y. Construction, application and biosafety of silver nanocrystalline chitosan wound dressing. Burns, 2008, 34(5): 623–628

    Article  Google Scholar 

  5. Sashiwa H, Aiba S I. Chemically modified chitin and chitosan as biomaterials. Progress in Polymer Science, 2004, 29(9): 887–908

    Article  Google Scholar 

  6. Muzzarelli R A A. Chitins and chitosans for the repair of wounded skin, nerve, cartilage and bone. Carbohydrate Polymers, 2009, 76(2): 167–182

    Article  Google Scholar 

  7. Chang J, Liu W, Han B, et al. Investigation of the skin repair and healing mechanism of N-carboxymethyl chitosan in seconddegree burn wounds. Wound Repair and Regeneration, 2013, 21(1): 113–121

    Article  Google Scholar 

  8. Zhou Y, Yang H, Liu X, et al. Potential of quaternizationfunctionalized chitosan fiber for wound dressing. International Journal of Biological Macromolecules, 2013, 52: 327–332

    Article  Google Scholar 

  9. Anitha A, Divya Rani V V, Krishna R, et al. Synthesis, characterization, cytotoxicity and antibacterial studies of chitosan, O-carboxymethyl and N,O-carboxymethyl chitosan nanoparticles. Carbohydrate Polymers, 2009, 78(4): 672–677

    Article  Google Scholar 

  10. Kim I Y, Seo S J, Moon H S, et al. Chitosan and its derivatives for tissue engineering applications. Biotechnology Advances, 2008, 26(1): 1–21

    Article  Google Scholar 

  11. Qin Y, Hu H, Luo A, et al. Effect of carboxymethylation on the absorption and chelating properties of chitosan fibers. Journal of Applied Polymer Science, 2006, 99(6): 3110–3115

    Article  Google Scholar 

  12. Upadhyaya L, Singh J, Agarwal V, et al. Biomedical applications of carboxymethyl chitosans. Carbohydrate Polymers, 2013, 91(1): 452–466

    Article  Google Scholar 

  13. Rinaudo M. Chitin and chitosan: Properties and applications. Progress in Polymer Science, 2006, 31(7): 603–632

    Article  Google Scholar 

  14. Kong X. Simultaneous determination of degree of deacetylation, degree of substitution and distribution fraction of–COONa in carboxymethyl chitosan by potentiometric titration. Carbohydrate Polymers, 2012, 88(1): 336–341

    Article  Google Scholar 

  15. Chen X G, Park H J. Chemical characteristics of O-carboxymethyl chitosans related to the preparation conditions. Carbohydrate Polymers, 2003, 53(4): 355–359

    Article  Google Scholar 

  16. Zhou Y, Shi L, Li F, et al. Preparation and characterization of carboxymethyl-functionalized chitosan fiber. Journal of Natural Fibers, 2015, 12(3): 211–221

    Article  Google Scholar 

  17. Muzzarelli R A A, Tanfani F, Emanuelli M, et al. N- (carboxymethylidene) chitosans and N-(carboxymethyl) chitosans: Novel chelating polyampholytes obtained from chitosan glyoxylate. Carbohydrate Research, 1982, 107(2): 199–214

    Article  Google Scholar 

  18. Lv J, Zhou Q, Liu G, et al. Preparation and properties of polyester fabrics grafted with O-carboxymethyl chitosan. Carbohydrate Polymers, 2014, 113: 344–352

    Article  Google Scholar 

  19. Sweeney I R, Miraftab M, Collyer G. Absorbent alginate fibres modified with hydrolysed chitosan for wound care dressings—II. Pilot scale development. Carbohydrate Polymers, 2014, 102: 920–927

    Article  Google Scholar 

  20. Zeng X, Sun Y X, Qu W, et al. Biotinylated transferrin/avidin/ biotinylated disulfide containing PEI bioconjugates mediated p53 gene delivery system for tumor targeted transfection. Biomaterials, 2010, 31(17): 4771–4780

    Article  Google Scholar 

  21. Li X, Kong X, Zhang Z, et al. Cytotoxicity and biocompatibility evaluation of N,O-carboxymethyl chitosan/oxidized alginate hydrogel for drug delivery application. International Journal of Biological Macromolecules, 2012, 50(5): 1299–1305

    Article  Google Scholar 

  22. Tao S, Gao S, Zhou Y, et al. Preparation of carboxymethyl chitosan sulfate for improved cell proliferation of skin fibroblasts. International Journal of Biological Macromolecules, 2013, 54: 160–165

    Article  Google Scholar 

  23. Zhou Z, Yan D, Cheng X, et al. Biomaterials based on N,N,Ntrimethyl chitosan fibers in wound dressing applications. International Journal of Biological Macromolecules, 2016, 89: 471–476

    Article  Google Scholar 

  24. Huang Y, Zhang L, Yang J, et al. Structure and properties of cellulose films reinforced by chitin whiskers. Macromolecular Materials and Engineering, 2013, 298(3): 303–310

    Article  Google Scholar 

  25. Ong S Y, Wu J, Moochhala SM, et al. Development of a chitosanbased wound dressing with improved hemostatic and antimicrobial properties. Biomaterials, 2008, 29(32): 4323–4332

    Article  Google Scholar 

  26. Dowling M B, Smith W, Balogh P, et al. Hydrophobicallymodified chitosan foam: description and hemostatic efficacy. The Journal of Surgical Research, 2015, 193(1): 316–323

    Article  Google Scholar 

  27. Upadhyaya L, Singh J, Agarwal V, et al. The implications of recent advances in carboxymethyl chitosan based targeted drug delivery and tissue engineering applications. Journal of Controlled Release, 2014, 186: 54–87

    Article  Google Scholar 

  28. Gong C, Wu Q, Wang Y, et al. A biodegradable hydrogel system containing curcumin encapsulated in micelles for cutaneous wound healing. Biomaterials, 2013, 34(27): 6377–6387

    Article  Google Scholar 

  29. Murakami K, Aoki H, Nakamura S, et al. Hydrogel blends of chitin/chitosan, fucoidan and alginate as healing-impaired wound dressings. Biomaterials, 2010, 31(1): 83–90

    Article  Google Scholar 

  30. Brugnerotto J, Lizardi J, Goycoolea F M, et al. An infrared investigation in relation with chitin and chitosan characterization. Polymer, 2001, 42(8): 3569–3580

    Article  Google Scholar 

  31. Zhao Y W, Liu L, Han X, et al. Preparation of N, Ocarboxymethyl chitosan with different substitutional degree and its application for hemostasis. Advanced Materials Research, 2013, 798–799: 1061–1066

    Article  Google Scholar 

  32. Kubota N, Tatsumoto N, Sano T, et al. A simple preparation of half N-acetylated chitosan highly soluble in water and aqueous organic solvents. Carbohydrate Research, 2000, 324(4): 268–274

    Article  Google Scholar 

  33. Patrulea V, Applegate L A, Ostafe V, et al. Optimized synthesis of O-carboxymethyl-N,N,N-trimethyl chitosan. Carbohydrate Polymers, 2015, 122: 46–52

    Article  Google Scholar 

  34. Zheng M, Han B, Yang Y, et al. Synthesis, characterization and biological safety of O-carboxymethyl chitosan used to treat Sarcoma 180 tumor. Carbohydrate Polymers, 2011, 86(1): 231–238

    Article  Google Scholar 

  35. Lim S H, Hudson S M. Application of a fiber-reactive chitosan derivative to cotton fabric as an antimicrobial textile finish. Carbohydrate Polymers, 2004, 56(2): 227–234

    Article  Google Scholar 

  36. Lim S H, Hudson S M. Review of chitosan and its derivatives as antimicrobial agents and their uses as textile chemicals. Journal of Macromolecular Science Part C: Polymer Reviews, 2003, 43(2): 223–269

    Article  Google Scholar 

  37. Lim S H, Hudson S M. Synthesis and antimicrobial activity of a water-soluble chitosan derivative with a fiber-reactive group. Carbohydrate Research, 2004, 339(2): 313–319

    Article  Google Scholar 

  38. Raafat D, von Bargen K, Haas A, et al. Insights into the mode of action of chitosan as an antibacterial compound. Applied and Environmental Microbiology, 2008, 74(12): 3764–3773

    Article  Google Scholar 

  39. Kong M, Chen X G, Xing K, et al. Antimicrobial properties of chitosan and mode of action: a state of the art review. International Journal of Food Microbiology, 2010, 144(1): 51–63

    Article  Google Scholar 

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Authors and Affiliations

  1. College of Marine Life Science, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China

    Dong Yan, Zhong-Zheng Zhou, Chang-Qing Jiang, Xiao-Jie Cheng, Ming Kong, Ya Liu, Chao Feng & Xi-Guang Chen

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  1. Dong Yan
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  2. Zhong-Zheng Zhou
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  3. Chang-Qing Jiang
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  4. Xiao-Jie Cheng
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  6. Ya Liu
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  8. Xi-Guang Chen
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Correspondence to Xi-Guang Chen.

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Yan, D., Zhou, ZZ., Jiang, CQ. et al. Sodium carboxymethylation-functionalized chitosan fibers for cutaneous wound healing application. Front. Mater. Sci. 10, 358–366 (2016). https://doi.org/10.1007/s11706-016-0353-9

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  • DOI: https://doi.org/10.1007/s11706-016-0353-9

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