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Optimization of microwave-assisted extraction for picroside I and picroside II from Picrorrhiza kurroa using Box-Behnken experimental design

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Abstract

The response surface methodology was employed to study the optimization of microwave-assisted extraction of picroside I and picroside II from Picrorrhiza kurroa Royle rhizomes. The effects of solid to solvent ratio, and extraction temperature, time and solvent on the yields of picroside I and picroside II have been investigated using Box-Behnken experimental design. The experimental data were fitted to second-order polynomial equations using multiple regression analysis and analyzed using the appropriate statistical method. By solving the regression equation and analyzing 3-D plots, the optimum extraction conditions were found to be: solid to solvent ratio, 10: 90 (w/v); temperature, 60 °C; and extraction time, 60 s. Under the optimal conditions, the yields of picroside I and picroside II are 41.23 and 6.12 mg·g−1 feed respectively, which are in good agreement with the predicted values. The ratio of solid to solvent significantly affects the yields of picroside I and picroside II. Application of microwaveassisted extraction of picroside I and picroside II from P. kurroa would dramatically reduce extraction time and solvent consumption.

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Authors and Affiliations

  1. Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, 431004, India

    Pravin Wakte, Ajit Patil, Bhusari Sachin, Munnaza Quazi, Shraddha Jabde & Devanand Shinde

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  1. Pravin Wakte
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  2. Ajit Patil
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  3. Bhusari Sachin
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  4. Munnaza Quazi
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  5. Shraddha Jabde
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  6. Devanand Shinde
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Correspondence to Pravin Wakte.

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Wakte, P., Patil, A., Sachin, B. et al. Optimization of microwave-assisted extraction for picroside I and picroside II from Picrorrhiza kurroa using Box-Behnken experimental design. Front. Chem. Sci. Eng. 8, 445–453 (2014). https://doi.org/10.1007/s11705-014-1458-2

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  • DOI: https://doi.org/10.1007/s11705-014-1458-2

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