Abstract
Purpose
Diabetes and obesity are associated with inflammasome-mediated low-grade, chronic inflammation that may induce pancreatic beta-cell dysfunction and apoptosis. We examined the effects of Roux-en-Y gastric bypass (RYGB) surgery on NOD-like receptor family, pyrin domain containing-3 (NLRP3) inflammasome-related genes from pancreatic islets of Zucker diabetic fatty rats.
Materials and Methods
Islets were collected from Zucker diabetic fatty sham control and RYGB, 30 days after surgery. We assessed expression of genes that regulate glucose metabolism and the NLRP3 inflammasome (NLRP3, caspase-1, IL-1β, IL-18, apoptosis-associated speck-like protein), IL-6, and monocyte chemoattractant protein-1.
Results
Gene expression for NLRP3 (p < 0.02), IL-1β (p < 0.04), and IL-6 (p < 0.01) was reduced by RYGB and positively correlated with change in body weight. IL-1β positively correlated with glucose AUC response.
Conclusion
Suppression of the NLRP3 inflammasome in pancreatic islets may contribute to improved glycemic control after RYGB.
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Acknowledgments
A.O.M. and J.P.K. developed the NLRP3 hypothesis. A.O.M. was primarily responsible for the sample and statistical analysis. A.O.M., A.M., O.D., and H.H. performed the data collection. A.O.M. and J.P.K. wrote the manuscript and all authors contributed to the data interpretation and editing the manuscript. J.P.K. takes the responsibility for the data integrity.
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Mocanu, A.O., Mulya, A., Huang, H. et al. Effect of Roux-en-Y Gastric Bypass on the NLRP3 Inflammasome in Pancreatic Islets from Zucker Diabetic Fatty Rats. OBES SURG 26, 3076–3081 (2016). https://doi.org/10.1007/s11695-016-2373-z
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DOI: https://doi.org/10.1007/s11695-016-2373-z