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Effects of Modified Biliopancreatic Diversion on Glucose Tolerance of GK Rats

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Abstract

Background

The purpose of this study was to investigate the hypoglycemic effect of new biliopancreatic diversion and duodenal–jejunal bypass in Goto–Kakizaki rats and observe effects of the new surgical procedure on the glucose tolerance of GK rats.

Methods

Twenty-four 10-week-old rats (SPF grade) were randomly divided into groups A, B, and C, each with eight rats. Group A underwent duodenal–jejunal bypass, group B underwent modified biliopancreatic diversion, and group C underwent a sham operation. Median rat body weight, fasting blood glucose, OGTT, and blood lipids were measured in fasting 1 week before surgery and 1, 2, 4, and 8 weeks after surgery. Changes in gastric inhibitory polypeptide, glucagon P-like peptide-1, and insulin levels were measured by ELISA 1 week before surgery and 8 weeks after surgery.

Results

Rats’ mean body weight in groups A and B decreased significantly from 368.025 ± 11.726 and 373.100 ± 9.859 g preoperatively to 345.750 ± 11.403 and 343.260 ± 12.399 g at the early postoperative stage (P < 0.05), and with statistically significant differences compared to the weight of rats in group C (P < 0.05). Comparisons between fasting blood glucose before surgery and 8 weeks after surgery revealed no significant differences between all three groups (P > 0.05). Glucose tolerance in groups A and B decreased from preoperative 21.175 ± 3.684 and 20.820 ± 1.671 mmol/L to postoperative 8.950 ± 0.580 and 10.500 ± 1.509 mmol/L, and both were better than that of group C (P < 0.001).

Conclusions

Both new biliopancreatic diversion and duodenal–jejunal bypass improve glucose tolerance of Goto–Kakizaki rats.

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References

  1. Buchwald H, Avidor Y, Braunwald E, et al. Bariatric surgery: a systematic review and meta-analysis. JAMA. 2004;292:1724–37.

    Article  PubMed  CAS  Google Scholar 

  2. Pories WJ, Swanson MS, MacDonald KG, et al. Who would have thought it? An operation proves to be the most effective therapy for adult-onset diabetes mellitus. Ann Surg. 1995;222(3):339–50.

    Article  PubMed  CAS  Google Scholar 

  3. Rubino F, Marescaux J. Effect of duodenal–jejunal exclusion in a non-obese animal model of type 2 diabetes: a new perspective for an old disease. Ann Surg. 2004;239:1–11.

    Article  PubMed  Google Scholar 

  4. Mason EE. Ilial transposition and enteroglucagon/GLP-1 in obesity (and diabetic?) surgery. Obes Surg. 1999;9:223–8.

    Article  PubMed  CAS  Google Scholar 

  5. Patriti A, Aisa M, Annetti C, et al. How the hindgut can cure type 2 diabetes. Ileal transposition improves glucose metabolism and beta-cell function in Goto–Kakizaki rats through an enhanced proglucagon gene expression and L-cell number. Surgery. 2007;142(1):74–85.

    Article  PubMed  Google Scholar 

  6. Weng S-G. Modified biliopancreatic diversion for GK rats: a proposal for a simpler technique and mechanism research. Obes Surg. 2012;22(6):997–8.

    Article  PubMed  Google Scholar 

  7. Hanefeld M, Temelkova-Kurktschiev T. The postprandial state and the risk of atherosclerosis. Diabet Med. 1997;14 Suppl 3:S6–S11.

    Article  PubMed  Google Scholar 

  8. Coutinho M, et al. The relationship between glucose and incident cardiovascular events. Diabetes Care. 1999;22(2):223–40.

    Article  Google Scholar 

  9. O’Keefe JH, Bell DS. Postprandial hyperglycemia/hyperlipidemia (postprandial dysmetabolism) is a cardiovascular risk factor. Am J Cardiol. 2007;100(5):899–904.

    Article  PubMed  Google Scholar 

  10. Speck M, Cho YM, Rubino F, et al. Duodenal–jejunal bypass protects GK rats from beta-cell loss and aggravation of hyperglycemia and increases enteroendocrine cells co-expressing GIP and GLP-1. Am J Physiol Endocrinol Metab. 2011;300(5):E923–32.

    Article  PubMed  CAS  Google Scholar 

  11. Maedler K, Spinas GA, Dyntar D, et al. Distinct effects of saturated and monounsaturated fatty acids on beta-cell turnover and function. Diabetes. 2001;50(1):69–76.

    Article  PubMed  CAS  Google Scholar 

  12. Listenberger LL, Ory DS, Schaffer JE. Palmitate-induced apoptosis can occur through a ceramide-independent pathway. J Biol Chem. 2001;276(18):14890–5.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We are thankful to the Fujian Medical University for providing place for housed animal. We also thank the First Hospital Affiliated of Fujian Medical University and Fujian Province Institute of Abdominal Surgery for providing place for our research.

Conflict of interest

The authors declared no conflict of interest.

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Authors and Affiliations

  1. Hepatopancreatobiliary Surgery Department, The First Affiliated Hospital of Fujian Medical University, Cha-Zhong Road, Fuzhou, 350005, People’s Republic of China

    Shan-geng Weng, Bin Zhang, Su Feng, Chang-guo Xu & Chun-zhong Lin

Authors
  1. Shan-geng Weng
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  2. Bin Zhang
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  3. Su Feng
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  4. Chang-guo Xu
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  5. Chun-zhong Lin
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Corresponding author

Correspondence to Shan-geng Weng.

Additional information

Supported by: the Fujian Provincial Natural Science Foundation, No. 2010J01152

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Weng, Sg., Zhang, B., Feng, S. et al. Effects of Modified Biliopancreatic Diversion on Glucose Tolerance of GK Rats. OBES SURG 23, 522–530 (2013). https://doi.org/10.1007/s11695-012-0830-x

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  • DOI: https://doi.org/10.1007/s11695-012-0830-x

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