Obesity Surgery

, Volume 20, Issue 5, pp 616–622

Vagal Sparing Surgical Technique but Not Stoma Size Affects Body Weight Loss in Rodent Model of Gastric Bypass

  • Marco Bueter
  • Christian Löwenstein
  • Hutan Ashrafian
  • Jacquelien Hillebrand
  • Stephen R. Bloom
  • Torsten Olbers
  • Thomas Lutz
  • Carel W. le Roux
Animal Research

DOI: 10.1007/s11695-010-0075-5

Cite this article as:
Bueter, M., Löwenstein, C., Ashrafian, H. et al. OBES SURG (2010) 20: 616. doi:10.1007/s11695-010-0075-5

Abstract

Background

The aim of this study was to evaluate whether gastric bypass with or without vagal preservation resulted in a different outcome.

Methods

Body weight, food intake and postprandial peptide YY (PYY) and glucagon-like peptide (GLP-1) levels were compared between gastric bypass (n = 55) and sham-operated rats (n = 27) in three groups. In group 1 (n = 17), the vagal nerve was not preserved, while in group 2 the vagal nerve was preserved during gastric bypass (n = 10). In group 3, gastric bypass rats (n = 28) were randomised for either one of the two techniques.

Results

Rats in which the vagal nerve was preserved during gastric bypass showed a lower body weight (p < 0.001) and reduced food intake (p < 0.001) compared to rats in which the vagal nerve was not preserved during the gastric bypass operation. Levels of PYY and GLP-1 were significantly increased after gastric bypass compared to sham-operated controls (p < 0.05), but there was no difference between gastric bypass rats with and without vagal preservation. Differences in food intake and body weight were not related to the size of the gastro-jejunostomy in gastric bypass rats. There were no signs of malabsorption or inflammation after gastric bypass.

Conclusion

We propose that the vagal nerve should be preserved during the gastric bypass operation as this might play an important role for the mechanisms that induce weight loss and reduce food intake in rats. In contrast, the gastro-jejunal stoma size was found to be of minor relevance.

Keywords

Gastric bypassRatsPara-oesophageal bundleVagal nerveLeft gastric vesselsWeight loss

Copyright information

© Springer Science + Business Media, LLC 2010

Authors and Affiliations

  • Marco Bueter
    • 1
    • 2
  • Christian Löwenstein
    • 3
  • Hutan Ashrafian
    • 1
  • Jacquelien Hillebrand
    • 4
  • Stephen R. Bloom
    • 1
  • Torsten Olbers
    • 1
  • Thomas Lutz
    • 3
  • Carel W. le Roux
    • 1
  1. 1.Imperial Weight Centre, Department of Investigative Medicine, Hammersmith HospitalImperial College LondonLondonUK
  2. 2.Department of SurgeryUniversity of WürzburgWürzburgGermany
  3. 3.Institute of Veterinary Physiology and Zürich Centre for Integrative Human Physiology, Vetsuisse FacultyUniversity of ZurichZurichSwitzerland
  4. 4.Swiss Federal Institute of TechnologyPhysiology and Behavior GroupSchwerzenbachSwitzerland