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Occurrence, genetic characterization and antimicrobial resistance of extended spectrum β-lactamase producing Escherichia coli isolated from Sürk samples, a traditional turkish cheese

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Abstract

This study was carried out to provide information regarding the presence of extended spectrum β-lactamase (ESBL) producing Escherichia coli isolated from Sürk cheese samples. For this purpose, ESBL-producing E. coli strains were isolated from 87 Sürk samples and isolates were screened for the common ESBL-types, presence of integrons and sul genes via PCR. All ESBL-producing E. coli strains were also allotted to phylogenetic groups and typed by PFGE. Twelve ESBL-producing E. coli (13.8 %) displayed eight distinct PFGE profiles and were exhibited resistance to tetracycline (41.7 %), trimethoprim-sulfamethoxazole (25 %), doxycycline (25 %) and chloramphenicol (16.7 %). Eleven isolates carried the bla CTX-M-15 gene (n = 7) and bla CTX-M-15 + bla TEM-1 genes (n = 4). Eleven isolates carried class1 and 2 integrons (intl1, 9 isolates and intl1 + intl2, two isolates) whereas four isolates carried sul1 (n = 2) and both sul1 + sul2 (n = 2) genes. The current study revealed a high rate of E. coli producing CTX-M-15 enzymes indicating a significant source for this organism in humans.

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Acknowledgments

Part of this study belonged to master thesis of MH and was received a grant from Mustafa Kemal University (BAP-14024). We gratefully thank Dr. Özlem Unaldi (Public Health Institution of Turkey, Ankara) for the PFGE analysis. We are also thankful to Prof. Dr. Ozkan Aslantas (Mustafa Kemal University, Hatay) for improving the manuscript.

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Authors and Affiliations

  1. Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Mustafa Kemal University, Antakya, Hatay, Turkey

    Cemil Kürekci

  2. Department of Food Engineering, Graduate School of Natural and Applied Sciences, Mustafa Kemal University, Antakya, Hatay, Turkey

    Müge Arkadaş

  3. Department of Food Engineering, Faculty of Agriculture, Mustafa Kemal University, Hatay, Turkey

    Yahya Kemal Avşar

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  1. Cemil Kürekci
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  2. Müge Arkadaş
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Correspondence to Cemil Kürekci.

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Kürekci, C., Arkadaş, M. & Avşar, Y.K. Occurrence, genetic characterization and antimicrobial resistance of extended spectrum β-lactamase producing Escherichia coli isolated from Sürk samples, a traditional turkish cheese. Food Measure 10, 709–714 (2016). https://doi.org/10.1007/s11694-016-9355-7

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