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Identification of mangrove endophytic fungus 1403 (Fusarium proliferatum) based on morphological and molecular evidence

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Abstract

A mangrove endophytic fungus 1403 isolated from the South China Sea Coast, which is able to produce griseofulvin and anthracenediones under submerged fermentation, was compared with Fusarium genus with the similar morphological characters such as elongated, microconidium-producing conidiophores, ovoid microconidia and straight to slightly curved macroconidia. It was found that the fungus 1403 resembles pathogenic F. verticillioides (teleomophy Gibberella moniliforme) in the production of false head or chains and abundant microconidia on the aerial mycelium, but different in the occasional formation of polyphialides with relatively long as well as short monophialides, in its typical coiled hyphae and mycelia fusion. Through maximum Parsimony and Bayesian analyses, the fungus 1403 was further compared with some similar Fusarium species. The results indicated that this endophyte was identified as Fusarium proliferatum based on the analyses of partial 18S and 28S rDNA genes, ITS region, and EF-1α gene.

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Authors and Affiliations

  1. School of Life Science, Sun Yat-sen University, Guangzhou, 510275, P. R. China

    Zhong-shan Cheng, Wen-cheng Tang, Yi Cai, Shi-feng Sun, Qi-jin Chen & Fang-hai Wang

  2. School of Pharmaceutical Science, Jinan University, Guangzhou, 510632, P.R. China

    Zhi-jian Su

  3. School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou, 510275, P.R. China

    Yong-cheng Lin & Zhi-gang She

  4. Department of Biology and Chemistry, City University of Hong Kong, Hongkong, China

    LLP Vrijmoed

Authors
  1. Zhong-shan Cheng
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  2. Wen-cheng Tang
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  3. Zhi-jian Su
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  4. Yi Cai
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  5. Shi-feng Sun
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  6. Qi-jin Chen
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  7. Fang-hai Wang
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  8. Yong-cheng Lin
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  9. Zhi-gang She
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  10. LLP Vrijmoed
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Corresponding authors

Correspondence to Qi-jin Chen or Fang-hai Wang.

Additional information

Foundation project: This research was supported partly by the Guangzhou Natural Science Foundation (Grant No. 2007Z3-EO581), the Guangdong Provincial Natural Science Foundation (Grant No. 2007A0200300001-7; 05003268), the Chinese High-Tech 863 Project (Grant No. 2006AA09Z422), and the National Natural Science Foundation of China (Grant No. 20572136)

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Cheng, Zs., Tang, Wc., Su, Zj. et al. Identification of mangrove endophytic fungus 1403 (Fusarium proliferatum) based on morphological and molecular evidence. Journal of Forestry Research 19, 219–224 (2008). https://doi.org/10.1007/s11676-008-0030-7

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  • DOI: https://doi.org/10.1007/s11676-008-0030-7

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