Protein Functionalized Nanodiamond Arrays
- YL LiuAffiliated withDepartment of Applied Chemistry, National Chiao Tung University
- , KW SunAffiliated withDepartment of Applied Chemistry, National Chiao Tung University Email author
Various nanoscale elements are currently being explored for bio-applications, such as in bio-images, bio-detection, and bio-sensors. Among them, nanodiamonds possess remarkable features such as low bio-cytotoxicity, good optical property in fluorescent and Raman spectra, and good photostability for bio-applications. In this work, we devise techniques to position functionalized nanodiamonds on self-assembled monolayer (SAMs) arrays adsorbed on silicon and ITO substrates surface using electron beam lithography techniques. The nanodiamond arrays were functionalized with lysozyme to target a certain biomolecule or protein specifically. The optical properties of the nanodiamond-protein complex arrays were characterized by a high throughput confocal microscope. The synthesized nanodiamond-lysozyme complex arrays were found to still retain their functionality in interacting with E. coli.
KeywordsNanodiamond Biosensor Self-assembled monolayer
- Protein Functionalized Nanodiamond Arrays
- Open Access
- Available under Open Access This content is freely available online to anyone, anywhere at any time.
Nanoscale Research Letters
- Online Date
- April 2010
- Online ISSN
- Springer New York
- Additional Links
- Self-assembled monolayer