Nano Express

Nanoscale Research Letters

, 5:1045

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Protein Functionalized Nanodiamond Arrays

  • YL LiuAffiliated withDepartment of Applied Chemistry, National Chiao Tung University
  • , KW SunAffiliated withDepartment of Applied Chemistry, National Chiao Tung University Email author 


Various nanoscale elements are currently being explored for bio-applications, such as in bio-images, bio-detection, and bio-sensors. Among them, nanodiamonds possess remarkable features such as low bio-cytotoxicity, good optical property in fluorescent and Raman spectra, and good photostability for bio-applications. In this work, we devise techniques to position functionalized nanodiamonds on self-assembled monolayer (SAMs) arrays adsorbed on silicon and ITO substrates surface using electron beam lithography techniques. The nanodiamond arrays were functionalized with lysozyme to target a certain biomolecule or protein specifically. The optical properties of the nanodiamond-protein complex arrays were characterized by a high throughput confocal microscope. The synthesized nanodiamond-lysozyme complex arrays were found to still retain their functionality in interacting with E. coli.


Nanodiamond Biosensor Self-assembled monolayer