Preparation and Sustained-Release Property of Triblock Copolymer/Calcium Phosphate Nanocomposite as Nanocarrier for Hydrophobic Drug
The P123/ACP nanocomposite with sizes less than 100 nm consisting of triblock copolymer P123 and amorphous calcium phosphate (ACP) has been prepared by using an aqueous solution containing CaCl2, (NH4)3PO4, and P123 at room temperature. The P123/ACP nanocomposite is used as the nanocarrier for hydrophobic drug ibuprofen, based on the combined advantages of both amphiphilic block copolymer and calcium phosphate delivery system. The P123/ACP nanocomposite has a much higher ibuprofen loading capacity (148 mg/g) than the single-phase calcium phosphate nanostructures. The drug release percentage of the P123/ACP nanocomposite in simulated body fluid reaches about 100% in a period of 156 h, which is much slower than that of single-phase calcium phosphate nanostructures. It is expected that the P123/ACP nanocomposite is promising for the application in the controlled delivery of hydrophobic drugs.
KeywordsNanocomposite P123 Calcium phosphate Hydrophobic drug Sustained release
Calcium phosphates exist throughout the body in the form of amorphous calcium phosphate as well as crystalline hydroxyapatite . They have been widely studied as fillers of bony tissues [2, 3], coatings of orthopedic implants , fillers of polymer bone cements , immovable phases for liquid chromatography of proteins , and carriers of cell cultures . Recently, calcium phosphates have been used to provide transfection of DNA and deliver drugs via surface decoration of microparticles [8–13], because calcium phosphates are naturally nontoxic as well as bioresorbable. Calcium phosphates are native to the body, and the problems of metabolism pathway can be greatly reduced compared to other delivery systems . One advantage of calcium phosphates as embedding materials is that they are hydrophilic by nature . Thus, drugs with little solubility in physiological liquids can be delivered through the blood stream to target tissues using calcium phosphates if the hydrophobic drugs can achieve an effective loading capacity.
Amphiphilic block copolymers have recently attracted significant attention in drug and gene delivery research [16–18], especially for hydrophobic drugs . Amphiphilic block copolymers self-associate in aqueous solution, forming a micellar structure with a diameter less than 100 nm. These micelles consist of a hydrophobic core surrounded by a shell of hydrophilic blocks. The core serves as a nonaqueous reservoir for hydrophobic drug, and the shell interacts with the biological environment and serves as a stabilizing interface between the hydrophobic core and the external medium. This characteristic enables both a high loading capacity of hydrophobic drugs and a long circulation time resulting from the steric hindrance due to the presence of a hydrophilic shell and the small scale of polymeric micelles . Besides, this characteristic also enables the coating of hydrophilic calcium phosphate on the hydrophilic shell.
Herein, we report the P123/ACP nanocomposite of a typical amphiphilic triblock copolymer P123 (PEO-PPO-PEO) and amorphous calcium phosphate (ACP) as the nanocarrier for hydrophobic drugs, which combines the advantages of both amphiphilic block copolymer and calcium phosphate delivery system. The structure of P123 block copolymer micelles consists of hydrophilic PEO chains attached to the surface of the hydrophobic PPO cores. And the amorphous calcium phosphate is coated on the micelles. A typical hydrophobic drug, ibuprofen, is used to investigate the drug loading and in vitro release behavior in simulated body fluid (SBF). The P123/ACP nanocomposite has higher ibuprofen loading capacity and superior sustained-release property compared with single-phase calcium phosphate delivery system, thus it is promising for the application in hydrophobic drug delivery.
Materials and Methods
Preparation of the P123/ACP Nanocomposite
The P123/ACP nanocomposite was prepared as follows: The reaction temperature was kept at room temperature. 0.100 g P123 was dissolved in 40 mL deionized water under magnetic stirring. An amount of 1.5 mL 0.5 M CaCl2 aqueous solution was added to the above solution under magnetic stirring. Then, 0.9 mL of 0.5 M (NH4)3PO4 aqueous solution was added and magnetically stirred for 30 min. The product was collected by centrifugation and washed by centrifugation-redispersion cycles with deionized water and alcohol three times, respectively.
Preparation of Single-Phase Calcium Phosphate Nanostructures
The single-phase calcium phosphate nanostructures were prepared as follows: The reaction temperature was kept at 37°C. The pH value of the reaction system was kept at 10. Solution A was prepared by dissolving 1.387 g CaCl2 in 75 mL deionized water. Solution B was prepared by dissolving 0.990 g (NH4)2HPO4 in 25 mL deionized water. Then, solution B was added to solution A. The product was collected by centrifugation and washed by centrifugation-redispersion cycles with deionized water and alcohol three times, respectively.
Characterization of Samples
The as-prepared samples were characterized using X-ray powder diffraction (XRD) (Rigaku D/max 2550 V, Cu Kα radiation,λ = 1.54178 Å), transmission electron microscopy (TEM) (JEOL JEM-2100F), selected-area electron diffraction (SAED), and energy dispersive spectroscopy (EDS). The Fourier transform infrared (FTIR) spectra were taken on a Nexus FTIR spectrometer (Thermo Nicolet, USA). The Brunauer–Emmett–Teller (BET) surface area was measured with an accelerated surface area and porosimetry system (ASAP 2010, USA). The ibuprofen concentrations were analyzed using a UV–Vis spectrophotometer (UV-2300, Techcomp) at a wavelength of 263 nm.
Drug Loading and In Vitro Drug Release
The typical drug loading and in vitro drug release experiments were performed as follows: 1 g of P123/ACP nanocomposite or single-phase calcium phosphate nanostructures was added into 40 mg mL−1 ibuprofen hexane solution. The suspension was shaken in a sealed vessel at 37°C for 24 h during which the evaporation of hexane was prevented. The sample with loaded drug was separated from the solution, dried, and compacted into disks (each disk 0.3 g) by a pressure of 4 MPa. Each disk was immersed into 200 mL of simulated body fluid (SBF) with pH 7.4 at 37°C with a constant shaking. The shaking device was a desk-type constant-temperature oscillator (THI-92A, China). The release medium (2 mL) was taken out for analysis by UV–Vis absorption spectroscopy at a wavelength of 263 nm at given time intervals and replaced with the same volume of fresh preheated SBF (37°C).
Results and Discussion
It has been reported that P123 self-assembles in water into spherical micelles at ambient temperatures . A P123 micelle consists of a hydrophobic core (PPO block) with a radius of 4.8 nm and a hydrophilic corona (PEO block) with a thickness of 4.6 nm, so that the diameter is around 18 nm . And generally spherical micelles aggregate together [23, 24]. These are consistent with the as-prepared P123/ACP nanocomposite. It is obvious that in the present reaction system, the amorphous calcium phosphate formed in a hydrophilic environment, thus leading to its coating on the hydrophilic corona (PEO block). As displayed in Fig. 1a, the as-prepared samples are composed of aggregated particles. Combining the results obtained from Figs. 1b and 2, one can see that these aggregated particles consist of calcium phosphate and P123. In short, it can be concluded that the P123/ACP nanocomposite formed as following: first, P123 self-assembled into spherical micelles and aggregated together; then amorphous calcium phosphate generated out of the hydrophilic PEO block when Ca2+ and PO43− were added into the aqueous solution and coated on the micelles.
Calcium phosphates, regardless of Ca:P ratio, crystallinity, or crystal phase, are relatively insoluble at physiological pH but have increasing solubility in the acidic environments [1, 15]. This pH-dependent solubility provides an advantage in the controlled drug delivery. And P123 is an important temperature responsive triblock copolymer. Thus, the P123/ACP nanocomposite may also be developed as both pH- and temperature-responsive drug delivery system. Future work will focus on the pH- and temperature-responsive property of these P123/ACP nanocomposites.
In summary, we have prepared the P123/ACP nanocomposite with sizes less than 100 nm by using an aqueous solution containing CaCl2, (NH4)3PO4, and P123 at room temperature. The P123/ACP nanocomposite has been explored as the nanocarrier for hydrophobic drug ibuprofen on the basis of the advantages of both amphiphilic block copolymer and calcium phosphate delivery system. The P123/ACP nanocomposite has a much higher ibuprofen loading capacity (148 mg/g) than the single-phase calcium phosphate nanostructures and has a prolonged drug release behavior in simulated body fluid (156 h), which is much slower than that of single-phase calcium phosphate nanostructures. It is expected that the P123/ACP nanocomposite is promising for the application in the controlled delivery of hydrophobic drugs.
Financial support from the Fund for Nano-Science and Technology from Science and Technology Commission of Shanghai (0852nm05800), the Program of Shanghai Subject Chief Scientist (07XD14031), and the National Natural Science Foundation of China (50772124, 50821004) is gratefully acknowledged.
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