Nano Express

Nanoscale Research Letters

, Volume 5, Issue 2, pp 302-307

Open Access This content is freely available online to anyone, anywhere at any time.

One-Pot Green Synthesis and Bioapplication of l-Arginine-Capped Superparamagnetic Fe3O4 Nanoparticles

  • Yongchao LaiAffiliated withKey Laboratory for Colloid & Interface Chemistry of Education Ministry, Department of Chemistry, Shandong University
  • , Weiwei YinAffiliated withKey Laboratory for Colloid & Interface Chemistry of Education Ministry, Department of Chemistry, Shandong University
  • , Jinting LiuAffiliated withKey Laboratory for Colloid & Interface Chemistry of Education Ministry, Department of Chemistry, Shandong University
  • , Rimo XiAffiliated withKey Laboratory for Colloid & Interface Chemistry of Education Ministry, Department of Chemistry, Shandong UniversityCollege of Pharmaceutical Sciences, Nankai University
  • , Jinhua ZhanAffiliated withKey Laboratory for Colloid & Interface Chemistry of Education Ministry, Department of Chemistry, Shandong University Email author 

Abstract

Water-soluble l-arginine-capped Fe3O4 nanoparticles were synthesized using a one-pot and green method. Nontoxic, renewable and inexpensive reagents including FeCl3, l-arginine, glycerol and water were chosen as raw materials. Fe3O4 nanoparticles show different dispersive states in acidic and alkaline solutions for the two distinct forms of surface binding l-arginine. Powder X-ray diffraction and X-ray photoelectron spectroscopy were used to identify the structure of Fe3O4 nanocrystals. The products behave like superparamagnetism at room temperature with saturation magnetization of 49.9 emu g−1 and negligible remanence or coercivity. In the presence of 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride, the anti-chloramphenicol monoclonal antibodies were connected to the l-arginine-capped magnetite nanoparticles. The as-prepared conjugates could be used in immunomagnetic assay.

Keywords

Magnetite Superparamagnetic Solvothermal Amino acid Nanocrystals