Nano Express

Nanoscale Research Letters

, Volume 4, Issue 12, pp 1428-1433

Open Access This content is freely available online to anyone, anywhere at any time.

Fabrication of Anti-human Cardiac Troponin I Immunogold Nanorods for Sensing Acute Myocardial Damage

  • Z. R. GuoAffiliated withThe Research Institute of Cardiovascular Disease, The First Affiliated Hospital of Nanjing Medical UniversityState Key Laboratory of Bioelectronics and Jiangsu Laboratory for Biomaterials and Devices, Southeast University
  • , C. R. GuAffiliated withThe Research Institute of Cardiovascular Disease, The First Affiliated Hospital of Nanjing Medical University
  • , X. FanAffiliated withState Key Laboratory of Bioelectronics and Jiangsu Laboratory for Biomaterials and Devices, Southeast University
  • , Z. P. BianAffiliated withThe Research Institute of Cardiovascular Disease, The First Affiliated Hospital of Nanjing Medical University
  • , H. F. WuAffiliated withThe Research Institute of Cardiovascular Disease, The First Affiliated Hospital of Nanjing Medical University
  • , D. YangAffiliated withThe Research Institute of Cardiovascular Disease, The First Affiliated Hospital of Nanjing Medical University
  • , N. GuAffiliated withState Key Laboratory of Bioelectronics and Jiangsu Laboratory for Biomaterials and Devices, Southeast University
  • , J. N. ZhangAffiliated withThe Research Institute of Cardiovascular Disease, The First Affiliated Hospital of Nanjing Medical University Email author 

Abstract

A facile, rapid, solution-phase method of detecting human cardiac troponin I for sensing myocardial damage has been described using gold nanorods-based biosensors. The sensing is demonstrated by the distinct change of the longitudinal surface plasmon resonance wavelength of the gold nanorods to specific antibody–antigen binding events. For a higher sensitivity, the aspect ratio of gold nanorods is increased up to ca 5.5 by simply adding small amount of HCl in seed-mediated growth solution. Experimental results show that the detecting limit of the present method is 10 ng/mL. Contrast tests reveal that these gold nanorods-based plasmonic biosensors hold much higher sensitivity than that of conventionally spherical gold nanoparticles.

Keywords

Gold nanorod Biosensor Surface modification Cardiac troponin I