Nano Express

Nanoscale Research Letters

, Volume 4, Issue 11, pp 1275-1285

Open Access This content is freely available online to anyone, anywhere at any time.

The Acute Liver Injury in Mice Caused by Nano-Anatase TiO2

  • Linglan MaAffiliated withMedical College of Soochow University
  • , Jinfang ZhaoAffiliated withMedical College of Soochow University
  • , Jue WangAffiliated withMedical College of Soochow University
  • , Jie LiuAffiliated withMedical College of Soochow University
  • , Yanmei DuanAffiliated withMedical College of Soochow University
  • , Huiting LiuAffiliated withMedical College of Soochow University
  • , Na LiAffiliated withMedical College of Soochow University
  • , Jingying YanAffiliated withMedical College of Soochow University
  • , Jie RuanAffiliated withMedical College of Soochow University
    • , Han WangAffiliated withMedical College of Soochow University
    • , Fashui HongAffiliated withMedical College of Soochow University Email author 

Abstract

Although it is known that nano-TiO2 or other nanoparticles can induce liver toxicities, the mechanisms and the molecular pathogenesis are still unclear. In this study, nano-anatase TiO2 (5 nm) was injected into the abdominal cavity of ICR mice for consecutive 14 days, and the inflammatory responses of liver of mice was investigated. The results showed the obvious titanium accumulation in liver DNA, histopathological changes and hepatocytes apoptosis of mice liver, and the liver function damaged by higher doses nano-anatase TiO2. The real-time quantitative RT-PCR and ELISA analyses showed that nano-anatase TiO2 can significantly alter the mRNA and protein expressions of several inflammatory cytokines, including nucleic factor-κB, macrophage migration inhibitory factor, tumor necrosis factor-α, interleukin-6, interleukin-1β, cross-reaction protein, interleukin-4, and interleukin-10. Our results also implied that the inflammatory responses and liver injury may be involved in nano-anatase TiO2-induced liver toxicity.

Keywords

Mice Nano-anatase TiO2 Liver Inflammatory cytokines Histopathological changes