Nano Express

Nanoscale Research Letters

, Volume 4, Issue 10, pp 1222-1229

Open Access This content is freely available online to anyone, anywhere at any time.

Micro/Nanoscale Parallel Patterning of Functional Biomolecules, Organic Fluorophores and Colloidal Nanocrystals

  • S. SabellaAffiliated withItalian Institute of Technology (IIT), National Nanotechnology Laboratory
  • , V. BrunettiAffiliated withNational Nanotechnology Laboratory of CNR-INFM, Scuola Superiore ISUFI, University of Salento
  • , G. VecchioAffiliated withNational Nanotechnology Laboratory of CNR-INFM, Scuola Superiore ISUFI, University of Salento
  • , A. Della TorreAffiliated withNational Nanotechnology Laboratory of CNR-INFM, Scuola Superiore ISUFI, University of Salento
  • , R. RinaldiAffiliated withNational Nanotechnology Laboratory of CNR-INFM, Scuola Superiore ISUFI, University of Salento
  • , R. CingolaniAffiliated withItalian Institute of Technology (IIT), National Nanotechnology Laboratory
  • , P. P. PompaAffiliated withItalian Institute of Technology (IIT), National Nanotechnology Laboratory Email author 

Abstract

We describe the design and optimization of a reliable strategy that combines self-assembly and lithographic techniques, leading to very precise micro-/nanopositioning of biomolecules for the realization of micro- and nanoarrays of functional DNA and antibodies. Moreover, based on the covalent immobilization of stable and versatile SAMs of programmable chemical reactivity, this approach constitutes a general platform for the parallel site-specific deposition of a wide range of molecules such as organic fluorophores and water-soluble colloidal nanocrystals.

Keywords

Molecular self-assembly Lithographic techniques DNA Green fluorescent proteins Colloidal nanocrystals