Nano Express

Nanoscale Research Letters

, Volume 4, Issue 7, pp 732-737

Open Access This content is freely available online to anyone, anywhere at any time.

New Method to Prepare Mitomycin C Loaded PLA-Nanoparticles with High Drug Entrapment Efficiency

  • Zhenqing HouAffiliated withResearch Center of Biomedical Engineering of Xiamen University, Material College of Xiamen University
  • , Heng WeiAffiliated withResearch Center of Biomedical Engineering of Xiamen University, Material College of Xiamen University
  • , Qian WangAffiliated withResearch Center of Biomedical Engineering of Xiamen University, Material College of Xiamen University
  • , Qian SunAffiliated withResearch Center of Biomedical Engineering of Xiamen University, Material College of Xiamen University
  • , Chunxiao ZhouAffiliated withResearch Center of Biomedical Engineering of Xiamen University, Material College of Xiamen University
  • , Chuanming ZhanAffiliated withResearch Center of Biomedical Engineering of Xiamen University, Material College of Xiamen University
  • , Xiaolong TangAffiliated withResearch Center of Biomedical Engineering of Xiamen University, Material College of Xiamen University
  • , Qiqing ZhangAffiliated withResearch Center of Biomedical Engineering of Xiamen University, Material College of Xiamen UniversityChinese Academy of Medical Sciences, Peking Union Medical College Institute of Biomedical Engineering Email author 

Abstract

The classical utilized double emulsion solvent diffusion technique for encapsulating water soluble Mitomycin C (MMC) in PLA nanoparticles suffers from low encapsulation efficiency because of the drug rapid partitioning to the external aqueous phase. In this paper, MMC loaded PLA nanoparticles were prepared by a new single emulsion solvent evaporation method, in which soybean phosphatidylcholine (SPC) was employed to improve the liposolubility of MMC by formation of MMC–SPC complex. Four main influential factors based on the results of a single-factor test, namely, PLA molecular weight, ratio of PLA to SPC (wt/wt) and MMC to SPC (wt/wt), volume ratio of oil phase to water phase, were evaluated using an orthogonal design with respect to drug entrapment efficiency. The drug release study was performed in pH 7.2 PBS at 37 °C with drug analysis using UV/vis spectrometer at 365 nm. MMC–PLA particles prepared by classical method were used as comparison. The formulated MMC–SPC–PLA nanoparticles under optimized condition are found to be relatively uniform in size (594 nm) with up to 94.8% of drug entrapment efficiency compared to 6.44 μm of PLA–MMC microparticles with 34.5% of drug entrapment efficiency. The release of MMC shows biphasic with an initial burst effect, followed by a cumulated drug release over 30 days is 50.17% for PLA–MMC–SPC nanoparticles, and 74.1% for PLA–MMC particles. The IR analysis of MMC–SPC complex shows that their high liposolubility may be attributed to some weak physical interaction between MMC and SPC during the formation of the complex. It is concluded that the new method is advantageous in terms of smaller size, lower size distribution, higher encapsulation yield, and longer sustained drug release in comparison to classical method.

Keywords

Mitomycin C PLA Nanoparticles Drug release