Nano Express

Nanoscale Research Letters

, Volume 4, Issue 7, pp 606-612

Open Access This content is freely available online to anyone, anywhere at any time.

Subcellular Localization of Thiol-Capped CdTe Quantum Dots in Living Cells

  • Yu ZhangAffiliated withDepartment of Optical Science and Engineering, Key Lab for Advanced Photonic Materials and Devices, Fudan University
  • , Lan MiAffiliated withDepartment of Optical Science and Engineering, Key Lab for Advanced Photonic Materials and Devices, Fudan University
  • , Rongling XiongAffiliated withDepartment of Optical Science and Engineering, Key Lab for Advanced Photonic Materials and Devices, Fudan University
  • , Pei-Nan WangAffiliated withDepartment of Optical Science and Engineering, Key Lab for Advanced Photonic Materials and Devices, Fudan University Email author 
  • , Ji-Yao ChenAffiliated withSurface Physics Laboratory (National Key Lab) and Department of Physics, Fudan University Email author 
  • , Wuli YangAffiliated withDepartment of Macromolecular Science and Key Lab of Molecular Engineering of Polymers, Fudan University
  • , Changchun WangAffiliated withDepartment of Macromolecular Science and Key Lab of Molecular Engineering of Polymers, Fudan University
  • , Qian PengAffiliated withDepartment of Pathology, The National Hospital-Norwegian Radium Hospital, University of Oslo

Abstract

Internalization and dynamic subcellular distribution of thiol-capped CdTe quantum dots (QDs) in living cells were studied by means of laser scanning confocal microscopy. These unfunctionalized QDs were well internalized into human hepatocellular carcinoma and rat basophilic leukemia cells in vitro. Co-localizations of QDs with lysosomes and Golgi complexes were observed, indicating that in addition to the well-known endosome-lysosome endocytosis pathway, the Golgi complex is also a main destination of the endocytosed QDs. The movement of the endocytosed QDs toward the Golgi complex in the perinuclear region of the cell was demonstrated.

Keywords

Cells Confocal microscopy Imaging Quantum dots Subcellular localization