Nano Express

Nanoscale Research Letters

, Volume 4, Issue 4, pp 287-295

Open Access This content is freely available online to anyone, anywhere at any time.

Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging

  • Yuqing GeAffiliated withDepartment of Biological Science and Medical Engineering, Jiangsu Laboratory for Biomaterials and Devices, State Key Laboratory of Bioelectronics
  • , Yu ZhangAffiliated withDepartment of Biological Science and Medical Engineering, Jiangsu Laboratory for Biomaterials and Devices, State Key Laboratory of Bioelectronics
  • , Shiying HeAffiliated withDepartment of Biological Science and Medical Engineering, Jiangsu Laboratory for Biomaterials and Devices, State Key Laboratory of Bioelectronics
  • , Fang NieAffiliated withDepartment of Radiology, Zhongda Hospital, Southeast University
  • , Gaojun TengAffiliated withDepartment of Radiology, Zhongda Hospital, Southeast University
  • , Ning GuAffiliated withDepartment of Biological Science and Medical Engineering, Jiangsu Laboratory for Biomaterials and Devices, State Key Laboratory of Bioelectronics Email author 

Abstract

Labeling of cells with nanoparticles for living detection is of interest to various biomedical applications. In this study, novel fluorescent/magnetic nanoparticles were prepared and used in high-efficient cellular imaging. The nanoparticles coated with the modified chitosan possessed a magnetic oxide core and a covalently attached fluorescent dye. We evaluated the feasibility and efficiency in labeling cancer cells (SMMC-7721) with the nanoparticles. The nanoparticles exhibited a high affinity to cells, which was demonstrated by flow cytometry and magnetic resonance imaging. The results showed that cell-labeling efficiency of the nanoparticles was dependent on the incubation time and nanoparticles’ concentration. The minimum detected number of labeled cells was around 104 by using a clinical 1.5-T MRI imager. Fluorescence and transmission electron microscopy instruments were used to monitor the localization patterns of the magnetic nanoparticles in cells. These new magneto-fluorescent nanoagents have demonstrated the potential for future medical use.

Keywords

Magnetic nanoparticle Fluorescence Chitosan Magnetic resonance imaging