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Mechanism of baicalin compatibility in chinese medicine formula Banxia Xiexin Decoction (半夏泻心汤) by pharmacokinetics and indirect competitive enzyme-linked immunosorbent assays in mice

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Abstract

Objective

To determine the effects of different formulations of Banxia Xiexin Decoction (半夏泻 心汤, BXD) on the pharmacokinetics of baicalin (BAL) in mice.

Methods

Pungent, bitter, and sweet components of BXD (totaling 7 Chinese herbs) were formulated into the following groups: K (bitter herbs), XK (pungent and bitter herbs), KG (bitter and sweet herbs), and BXD (all 7 herbs) groups. These different formulations were administered intragastrically in mice, and blood was collected via the tail vein for continuous monitoring. BAL, which is a main active constituent in Scutellaria baicalensis Georgi., was detected in this study. Indirect competitive enzyme-linked immunosorbent assays (icELISAs) based on anti-BAL-monoclonal antibodies were employed to determine BAL concentrations in each group.

Results

The concentrations of BAL in blood samples from mice in the K and XK groups were lower than those in other groups. In all groups, BAL concentrations peaked at around 1–1.5 h and again at 5–7 h. There were no significant differences in the timing of peak BAL concentrations between groups. However, the peak concentrations and area under curve (AUC)0–36 h in the KG and BXD groups were almost 3 times of those in the K and XK groups.

Conclusions

Differing compatibilities of BXD caused dissimilar pharmacokinetics of BAL. Moreover, we demonstrated a method for the continuous detection of blood concentrations of Chinese medicines in mice, and icELISA may be a feasible technique for the study of pharmcokinetic mechanisms of Chinese medicine.

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References

  1. Yao Y, Zhang X, Wang Z, Zheng C, Li P, Huang C, et al. Deciphering the combination principles of traditional Chinese medicine from a systems pharmacology perspective based on Ma-huang Decoction. J Ethnopharmacol 2013;150:619–638.

    Article  PubMed  Google Scholar 

  2. Wang S, Hu Y, Tan W, Wu X, Chen R, Cao J, et al. Compatibility art of traditional Chinese medicine: from the perspective of herb pairs. J Ethnopharmacol 2012;143:412–423.

    Article  PubMed  Google Scholar 

  3. Qu HH, Zhao Y, Wang QG. A new thought of compound compatibility mechanism based on active small molecules monoclonal antibodies in herbs. Chin J Integr Tradit West Med (Chin) 2012;32:1416–1420.

    Google Scholar 

  4. Chen G, Lu F, Xu L, Dong H, Yi P, Wang F, et al. The antidiabetic effects and pharmacokinetic profi les of berberine in mice treated with Jiao-Tai-Wan and its compatibility. Phytomedicine 2013;20:780–786.

    Article  CAS  PubMed  Google Scholar 

  5. Zheng Q, Yue PF, Wu B, Hu PY, Wu ZF, Yang M. Pharmacokinetics comparative study of a novel Chinese traditional herbal formula and its compatibility. J Ethnopharmacol 2011;137:221–225.

    Article  PubMed  Google Scholar 

  6. Yang Y, Han FM, Du P, Chen Y. Pharmacokinetics of gastrodin from compound Tianma Granule in rats. ACTA Pharm Sin (Chin) 2010;45:484–488.

    CAS  Google Scholar 

  7. Zuo F, Zhou ZM, Zhang Q, Mao D, Xiong YL, Wang YL, et al. Pharmacokinetic study on the multi-constituents of Huangqin-Tang Decoction in rats. Biol Pharm Bull 2003;26:911–919.

    Article  CAS  PubMed  Google Scholar 

  8. Akao T, Sato K, Hanada M. Hepatic contribution to a marked increase in the plasma concentration of baicalin after oral administration of its aglycone, baicalein, in multidrug resistance-associated protein 2-defi cient rat. Biol Pharm Bull 2009;32:2079–2082.

    Article  CAS  PubMed  Google Scholar 

  9. Tian X, Cheng ZY, Jin H, Gao J, Qiao HL. Inhibitory effects of baicalin on the expression and activity of CYP3A induce the pharmacokinetic changes of midazolam in rats. Evid Based Complement Alternat Med 2013;2013:179643.

    PubMed  PubMed Central  Google Scholar 

  10. Song EF, Sun WL, Mei SS. Research progress of Banxia-Xiexin Decoction. Harbin: The Fourth Chinese Academic Schools Exchange Conference; 2012.

    Google Scholar 

  11. Zuo F, Zhou ZM, Yan MZ, Liu ML, Xiong YL, Zhang Q, et al. Metabolism of constituents in Huangqin-Tang, a prescription in traditional Chinese medicine, by human intestinal flora. Biol Pharm Bull 2002;25:558–563.

    Article  CAS  PubMed  Google Scholar 

  12. Zhao Y, Zhang QM, Wang QG. Discussion on characteristics of classical Chinese prescription compatibility theory. Forum Tradit Chin Med (Chin) 2004;19(2):7–8.

    Google Scholar 

  13. Lai MY, Hsiu SL, Chen CC, Hou YC, Chao PD. Urinary pharmacokinetics of baicalein, wogonin and their glycosides after oral administration of Scutellariae Radix in humans. Biol Pharm Bull 2003;26:79–83.

    Article  CAS  PubMed  Google Scholar 

  14. Su X, Qu HH, Zhao Y, Sun H, Sun Y, Wang XQ, et al. Establishment of quick enzyme-linked immunosorbent assay with monoclonal antibody against baicalin. Chin J Pharm Anal 2013;33:946–950.

    CAS  Google Scholar 

  15. Qu HH, Zhao Y, Wang XQ, Yang AL, Li YF, Lu JQ, et al. Synthesis and identifi cation of baicalin artifi cial antigen. J Beijing Univ Tradit Chin Med (Chin) 2010;33:606–609.

    CAS  Google Scholar 

  16. Zhao Y, Qu HH, Wang QG. Problems and prospects on the compatibility of complex prescription in the experimental study. Chin J Exp Tradit Med Formul (Chin) 2003;9:51–54.

    Google Scholar 

  17. Zhang HM, Song JZ, Tan HS, Xu HX, Li SL, Chen SL. From traditional decoction to modern granule: perspectives and prospects of Chinese medicine dispensing granules. World Sci Technol Modern Tradit Chin Med Mater Med (Chin) 2012;14:1740–1753.

    Google Scholar 

  18. Wei FH, Wang YH, Luo JB. Research situation of pharmacokinetic multimodal phenomenon. Chin Pharm J (Chin) 2005;40:1772–1774.

    CAS  Google Scholar 

  19. Zhong XG, Cheng FF, Wang QG, Li YH, Zhao Y, Chen M. Clinical and basic research ideas on modern application of classical prescriptions. J Tradit Chin Med (Chin) 2011;52:1640–1642.

    Google Scholar 

  20. Xu JK, Zhang WK, Zhao Y, Qu HH, Wang QG, eds. To investigate the regularity of Chinese prescription compatibility on the view of pharmacokinetics space-time. Kunming: Zhongjing Theory Branch China Association Chinese Medicine; 2012:4.

    Google Scholar 

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Authors and Affiliations

  1. Center of Scientific Experiment, Beijing University of Chinese Medicine, Beijing, 100029, China

    Hui-hua Qu  (屈会化)

  2. School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, 100029, China

    Bao-ping Qu  (屈保平), Shu-chen Liu  (刘姝晨), Gao-feng Qin  (秦高凤), Hui Kong  (孔 慧), Yan Zhao  (赵 琰) & Qing-guo Wang  (王庆国)

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  1. Hui-hua Qu  (屈会化)
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  2. Bao-ping Qu  (屈保平)
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  3. Shu-chen Liu  (刘姝晨)
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  4. Gao-feng Qin  (秦高凤)
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  5. Hui Kong  (孔 慧)
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  6. Yan Zhao  (赵 琰)
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  7. Qing-guo Wang  (王庆国)
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Corresponding author

Correspondence to Yan Zhao  (赵 琰).

Additional information

Supported by the National Natural Science Foundation of China (No. 81274043 and No. 81373542) and the Classical Prescription Basic Research Team of Beijing University of Chinese Medicine

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Qu, Hh., Qu, Bp., Liu, Sc. et al. Mechanism of baicalin compatibility in chinese medicine formula Banxia Xiexin Decoction (半夏泻心汤) by pharmacokinetics and indirect competitive enzyme-linked immunosorbent assays in mice. Chin. J. Integr. Med. (2016). https://doi.org/10.1007/s11655-016-2447-8

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  • DOI: https://doi.org/10.1007/s11655-016-2447-8

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