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Effects of serum containing Chinese medicine Sanpi Pingwei (散癖平胃) formula on proliferation and apoptosis of human SGC-7901 cells

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Abstract

Objective

To investigate the effects of serum containing Chinese medicine (CM) Sanpi Pingwei (散癖平胃, SPPW) formula on the proliferation and apoptosis of human SGC-7901 cells and the possible mechanism.

Methods

Serum containing CM SPPW formula (SPPW serum) was prepared by a serum pharmacology method. Human SGC-7901 cells were incubated with SPPW serum at three different concentrations and with the anticancer drug 5-fluorouracil (5-FU), respectively. Cell proliferation was assessed by MTT assay, and cell apoptosis was detected by flow cytometry assay. Real-time quantitative polymerase chain reaction (RT-PCR) and Western blot assay were employed to confirm the expressions of Bcl-2, Bax and p53 in SGC-7901 cells at mRNA and protein levels, respectively.

Results

SPPW serum suppressed the proliferation of SGC-7901 cells in a time- and dose-dependent manner. The colony forming rate of negative control was 48.2%, while those in the three SPPW serum groups and the 5-FU group decreased significantly (P<0.01). The number of colony forming units in the SPPW high dosage group was significantly smaller than that in the 5-FU group (P<0.01). MTT assay showed that SPPW serum restrained the proliferation of SGC-7901 cells, and the inhibition rate increased significantly in a dose-dependent manner. Annexin V/PI Assay suggested that SPPW serum induced the apoptosis of SGC-7901 cells significantly. RT-PCR and western blot assay indicated that SPPW serum upregulated the protein and mRNA expression levels of Bax and p53 in SGC-7901 cells, but downregulated the protein and mRNA expressions of Bcl-2.

Conclusions

SPPW formula inhibits the proliferation of SGC-7901 cells in vitro and induces the cell apoptosis. It plays an anticancer role by regulating the expressions of Bax, p53 and Bcl-2 in SGC-7901 cells.

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Authors and Affiliations

  1. Department of Gastroenterology, the Second Affiliated Hospital, Xi’an Jiaotong University, Xi’an, 710004, China

    Xiao-yan Dang  (党晓燕), Lei Dong  (董 蕾), Hai-tao Shi  (史海涛) & Bai-cang Zou  (邹百仓)

  2. Department of Emergency, the Second Affiliated Hospital, Xi’an Jiaotong University, Xi’an, 710004, China

    Xiao-yan Dang  (党晓燕)

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  1. Xiao-yan Dang  (党晓燕)
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  2. Lei Dong  (董 蕾)
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  3. Hai-tao Shi  (史海涛)
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  4. Bai-cang Zou  (邹百仓)
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Correspondence to Lei Dong  (董 蕾).

Additional information

Supported by “13115” Program of Shaanxi Province (No. 2007ZDKG-73)

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Dang, Xy., Dong, L., Shi, Ht. et al. Effects of serum containing Chinese medicine Sanpi Pingwei (散癖平胃) formula on proliferation and apoptosis of human SGC-7901 cells. Chin. J. Integr. Med. 19, 119–126 (2013). https://doi.org/10.1007/s11655-012-1208-6

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  • DOI: https://doi.org/10.1007/s11655-012-1208-6

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