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The absorption and transport of magnolol in Caco-2 cell model

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Abstract

Objective

To investigate the absorption and transport mechanism of magnolol in Caco-2 cell model.

Methods

A human intestinal epithelial cell model Caco-2 cell in vitro cultured was applied to study the absorption and transport of magnolol, the effects of time, donor concentration, P-gp inhibitor verapamil, pH and temperature on the absorption and transport of magnolol were investigated. The determination of magnolol was performed by high performance liquid chromatography, then the values of apparent permeability coefficient (P app ) and P ratio Basolateral-to-Apical (BL-to-AP)/Apical-to-Basolateral (AP-to-BL) were calculated.

Results

In Caco-2 cell model, comparing the amounts of transport of AP-to-BL and BL-to-AP, the latter was larger. At the same donor concentration, either the amounts of transport of AP-to-BL or BL-to-AP increased with increase in donor concentration and incubation time. Verapamil could significantly improve the amounts of transport of AP-to-BL. The transport of AP-to-BL and BL-to-AP depended on temperature, and there was no significant effect of pH on the transport of AP-to-BL.

Conclusion

Magnolol could be transported through the intestinal mucosa via a passive diffusion mechanism primarily, coexisting with a carrier-mediated transport, at the same time, the efflux mechanism could be involved.

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Authors and Affiliations

  1. Research Center of New Drug Development, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China

    An-guo Wu  (吴安国), Bao Zeng  (曾 宝), Meng-qiu Huang  (黄孟秋), Sheng-mei Li  (李生梅), Jian-nan Chen  (陈建南) & Xiao-ping Lai  (赖小平)

  2. Research Institute of Mathematical Engineering of Guangzhou University of Chinese Medicine in Dongguan, Dongguan, Guangdong Province, 523808, China

    An-guo Wu  (吴安国), Bao Zeng  (曾 宝) & Xiao-ping Lai  (赖小平)

Authors
  1. An-guo Wu  (吴安国)
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  2. Bao Zeng  (曾 宝)
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  3. Meng-qiu Huang  (黄孟秋)
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  4. Sheng-mei Li  (李生梅)
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  5. Jian-nan Chen  (陈建南)
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  6. Xiao-ping Lai  (赖小平)
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Corresponding author

Correspondence to Bao Zeng  (曾 宝).

Additional information

Supported by the National Key Technology Program (No. 2006BAI11B08-04)

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Wu, Ag., Zeng, B., Huang, Mq. et al. The absorption and transport of magnolol in Caco-2 cell model. Chin. J. Integr. Med. 19, 206–211 (2013). https://doi.org/10.1007/s11655-012-1098-7

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  • DOI: https://doi.org/10.1007/s11655-012-1098-7

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