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Statistical considerations for in vitro research: I — Birth of an idea to collecting data

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Summary

This manuscript is the first of two that discuss statistical methods applicable to in vitro research. This paper focuses on topics to consider when planning experiments and provides examples of several experimental designs that are suitable for plant tissue culture and biotechnology projects. Use of factorial treatment designs and designing sequential experiments are also discussed. The second paper deals with data handling. Several key references are provided at the end of each manuscript as sources of additional information for readers’ perusal.

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References

  • Cochran, W. G.; Cox, G. M. Experimental designs. New York: John Wiley & Sons; 1957:376–438.

    Google Scholar 

  • Compton, M. E. Statistical methods suitable for the analysis of plant tissue culture data. Plant Cell Tissue Organ Cult. 37:217–242; 1994.

    Google Scholar 

  • Compton, M. E. A revised protocol for shoot organogenesis from watermelon cotyledons. In Vitro Cell. Dev. Biol. Plant 34:45-A; 1998.

  • Kempthorne, O. The design and analysis of experiments. Malabar, FL: Robert E. Krieger Publishing Co.; 1973.

    Google Scholar 

  • Kuklin, A. I; Trigiano, R. N.; Sanders, W. L.; Conger, B. V. Incomplete block design in plant tissue culture research. J. Plant Tissue Cult. Methods 15:204–209; 1993.

    Article  Google Scholar 

  • Lentner, M.; Bishop, T. Experimental design and analysis. Blacksburg, VA: Valley Book Company; 1986.

    Google Scholar 

  • Little, T. M.; Hills, F. J. Agricultural experimentation: design and analysis. New York: John Wiley and Sons, Inc.; 1978.

    Google Scholar 

  • Mize, C. W.; Chun, Y. W. Analyzing treatment means in plant tissue culture research. Plant Cell Tissue Organ Cult. 13:201–217; 1988.

    Article  Google Scholar 

  • Mize, C. W.; Winistorfer, P. M. Application of subsampling to improve precision. Wood Sci. 15:14–18; 1982.

    Google Scholar 

  • Mize, C. W.; Koehler, K.; Compton, M. E. Statistical considerations for in vitro research: II—Data to presentation. In Vitro Cell. Dev. Biol. Plant. 35:122–126; 1999.

    CAS  Google Scholar 

  • Zar, J. H. Biostatistical analysis. 2nd ed. Englewood Cliffs, NJ: Prentice-Hall, Inc.; 1984.

    Google Scholar 

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Compton, M.E., Mize, C.W. Statistical considerations for in vitro research: I — Birth of an idea to collecting data. In Vitro Cell.Dev.Biol.-Plant 35, 115–121 (1999). https://doi.org/10.1007/s11627-999-0020-2

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  • DOI: https://doi.org/10.1007/s11627-999-0020-2

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