In Vitro Cellular & Developmental Biology - Plant

, Volume 49, Issue 2, pp 207–215

In vitro mycorrhization of the rubber tree Hevea brasiliensis Müll Arg

Authors

  • Tiffany Sosa-Rodriguez
    • Earth and Life Institute, Applied Microbiology, MycologyUniversité catholique de Louvain
  • Hervé Dupré de Boulois
    • Earth and Life Institute, Applied Microbiology, MycologyUniversité catholique de Louvain
  • Françoise Granet
    • Manufacture Française des Pneumatiques MICHELIN ZI Ladoux
  • Sylvie Gaurel
    • Manufacture Française des Pneumatiques MICHELIN ZI Ladoux
  • Luz-Marina Melgarejo
    • Departamento de Biología, Laboratorio de Fisiología VegetalUniversidad Nacional de Colombia
  • Marc-Philippe Carron
    • UPR34 Systèmes de PérennesCIRAD
    • Earth and Life Institute, Applied Microbiology, MycologyUniversité catholique de Louvain
Plant Tissue Culture

DOI: 10.1007/s11627-012-9485-5

Cite this article as:
Sosa-Rodriguez, T., Dupré de Boulois, H., Granet, F. et al. In Vitro Cell.Dev.Biol.-Plant (2013) 49: 207. doi:10.1007/s11627-012-9485-5

Abstract

In vitro cultivation systems of arbuscular mycorrhizal fungi are useful tools to study the interaction between plants and their fungal symbiont, and also to develop new biotechnologies. Plantlets of the latex-producing species Hevea brasiliensis clone PB 260 were grown in a dense extraradical mycelium network of the arbuscular mycorrhizal fungus Rhizophagus irregularis MUCL 41833 developed from a mycelium donor plant (Medicago truncatula A17). The factors indole-3-butyric acid (IBA), 2-morpholineoethanesulfonic acid monohydrate (MES) buffer, and carbon dioxide (CO2) were tested on root development and colonization by the fungus. No colonization was observed in the presence of plantlets pre-treated with IBA. The highest levels of root colonization were obtained when plantlets were mycorrhized under a high CO2 concentration (1,000 μmol mol−1) with MES (10 mM) added to the growth medium. Widespread root colonization (with presence of arbuscules, intraradical mycelium, and spores/vesicles) was predominantly observed in newly produced roots. Therefore, it appears essential to improve root initiation and growth for improving in vitro mycorrhization of H. brasiliensis. We demonstrated the potential of the “mycelium donor plant” in vitro culture system to produce colonized H. brasiliensis plantlets before their transfer to ex vitro conditions.

Keywords

Natural rubberArbuscular mycorrhizal fungiMycelium networkAutotrophyIn vitro culture

Copyright information

© The Society for In Vitro Biology 2013