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Effects of flask sealing and growth regulators on in vitro propagation of neem (Azadirachta indica A. Juss.)

  • Plant Tissue Culture
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Abstract

In vitro propagation of neem (Azadirachta indica A. Juss.) may offer an efficient alternative to seed propagation of this species. For optimization of in vitro propagation, different basal salt formulations, growth regulators, and culture container sealants (polytetrafluoroethylene hydrophobic membranes [PTFE]) were evaluated. Nodal segments cultured on Murashige and Skoog (MS) medium showed the highest shoot formation per explant (1.67). Explants cultured in flasks containing MS medium with 0.5 mg L−1 benzyladenine, 0.5 mg L−1 kinetin, and 0.05 mg L−1 naphthaleneacetic acid, and sealed with two PTFE membranes, produced the highest number of shoots (4.04). In contrast, explants cultured in flasks without membranes showed leaf chlorosis and senescence. For plant recovery, regenerants were acclimatized in a substrate of coconut fiber and eucalyptus bark (1:1) and showed 80% survival. Our results indicated that the use of flasks with vents was beneficial for in vitro propagation of this important plant.

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Acknowledgments

The authors would like to acknowledge the CNPq (National Council of Technological and Scientific Development, Brazil) for the scholarship to MR.

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Authors and Affiliations

  1. Departamento de Biologia Vegetal, Laboratório de Cultura de Tecidos/BIOAGRO, Universidade Federal de Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil

    Marcelo Rodrigues, Thales H. F. Costa & Wagner Campos Otoni

  2. Centro de Tecnologia Agropecuária, Instituto Sócioambiental e dos Recursos Hídricos, Universidade Federal Rural da Amazônia, Av. Tancredo Neves 2501, 66077-530, Belém, Pará, Brazil

    Reginaldo Alves Festucci-Buselli

  3. Departamento de Biologia Vegetal, Laboratório de Anatomia Vegetal, Universidade Federal de Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil

    Luzimar Campos Silva

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  1. Marcelo Rodrigues
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  2. Thales H. F. Costa
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  3. Reginaldo Alves Festucci-Buselli
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  4. Luzimar Campos Silva
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  5. Wagner Campos Otoni
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Correspondence to Wagner Campos Otoni.

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Editor: John Finer

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Rodrigues, M., Costa, T.H.F., Festucci-Buselli, R.A. et al. Effects of flask sealing and growth regulators on in vitro propagation of neem (Azadirachta indica A. Juss.). In Vitro Cell.Dev.Biol.-Plant 48, 67–72 (2012). https://doi.org/10.1007/s11627-011-9398-8

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