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Extracellular localization of napin in the embryogenic tissues of Brassica napus spp. oleifera

  • Developmental Biology
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Abstract

Napin, a storage protein, has been reported to be transcribed abundantly during the pre-embryogenic stage and associated with the induction of Brassica napus secondary embryogenesis. In this study, we studied the distribution pattern of napin in the winter oilseed rape embryogenic tissue in comparison to that of the non-embryogenic tissue using the indirect immunofluorescence localisation coupled with the ultrastructural immunogold labelling techniques. Immunolocalisation studies revealed that the extracellular matrix layer outside the outer epidermal cell wall of B. napus embryogenic tissues contained napin. This is the first study to report the extracellular localisation of napin. In addition, we have also further characterised the expression pattern of Eg1 that encodes for napin in the B. napus embryogenic tissue.

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Acknowledgement

We are grateful to Ms. Janet Powell (Multi-Imaging Centre, Department of Physiology Development and Neuroscience, Anatomy Building, University of Cambridge, UK) for the technical assistance.

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Authors and Affiliations

  1. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

    Parameswari Namasivayam

  2. Multi-Imaging Centre, Department of Physiology Development and Neuroscience, Anatomy Building, University of Cambridge, Downing Street, Cambridge, CB2 3EA, UK

    Jeremy Skepper

  3. Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, CB2 3EA, UK

    David Hanke

Authors
  1. Parameswari Namasivayam
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  2. Jeremy Skepper
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  3. David Hanke
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Correspondence to Parameswari Namasivayam.

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Editor: Gregory C. Philips

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Namasivayam, P., Skepper, J. & Hanke, D. Extracellular localization of napin in the embryogenic tissues of Brassica napus spp. oleifera . In Vitro Cell.Dev.Biol.-Plant 44, 273–281 (2008). https://doi.org/10.1007/s11627-008-9128-z

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  • DOI: https://doi.org/10.1007/s11627-008-9128-z

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