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Development of an Atlantic salmon heart endothelial cell line (ASHe) that responds to lysophosphatidic acid (LPA)

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Abstract

As diseases and abnormalities of the heart can interfere with the aquaculture of Atlantic salmon, the heart was investigated as a source of cell lines that could be used to study the cellular basis of these conditions. An Atlantic salmon heart endothelial cell line, ASHe, was developed and characterized for growth properties, endothelial cell characteristics, and responsiveness to lysophosphatidic acid (LPA). AHSe cells stained negative for senescence associated ß-galactosidase and grew well in 10 and 20% FBS/L15 at high cell density, but not in L15 medium supplemented with calf serum. It displayed many endothelial cell-like characteristics including a cobblestone morphology, capillary-like structures formation on Matrigel, and expression of von Willebrand factor and endothelial cell-related tight junction proteins ZO-1, claudin 3, and claudin 5. ASHe cells responded to the cardiovascular modulator, LPA, in two contrasting ways. LPA at 5 and 25 μM inhibited the ability of ASHe cells to heal a wound but stimulated their proliferation, especially as evaluated by colony formation in low-density cultures. The enhancement of proliferation by LPA parallels what has been observed previously in mammalian endothelial cell cultures exposed to LPA, whereas the LPA slowing of ASHe cell migration contrasted with the LPA-enhanced migration of some mammalian cells. Therefore, this cell line is a potentially useful model for future comparative studies on piscine and mammalian cardiovascular cell biology and for studies on diseases of Atlantic salmon in aquaculture.

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Acknowledgments

The authors would like to thank the Ontario Ministry of Natural Resources (OMNR) Normandale Fish Culture Station for providing the Atlantic salmon fish used in this work. This work was funded by a Natural Science and Engineering Research Council (NSERC) of Canada Collaborative Research and Development Grant and Elanco Animal Health.

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Authors and Affiliations

  1. Department of Biology, University of Waterloo, Waterloo, ON, Canada

    Phuc H. Pham, Elizabeth J. H. Tan & Niels C. Bols

  2. Department of Medical Physics and Applied Radiation Sciences, McMaster University, Hamilton, ON, Canada

    Nguyen T. K. Vo

  3. Department of Fisheries and Aquaculture, Vancouver Island University, Nanaimo, BC, Canada

    Spencer Russell

  4. Elanco Animal Health, PEI, Victoria, Canada

    Ginny Jones

  5. Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada

    John S. Lumsden

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  1. Phuc H. Pham
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Pham, P.H., Vo, N.T.K., Tan, E.J.H. et al. Development of an Atlantic salmon heart endothelial cell line (ASHe) that responds to lysophosphatidic acid (LPA). In Vitro Cell.Dev.Biol.-Animal 53, 20–32 (2017). https://doi.org/10.1007/s11626-016-0077-2

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