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Human umbilical cord-derived MSC culture: the replacement of animal sera with human cord blood plasma

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Abstract

Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) hold great potential for their therapeutic use in various clinical diseases. Many publications have reported on human blood-derived alternatives to animal serum for culturing mesenchymal stem cells, such as human serum, allogenic umbilical cord blood serum, and human platelet derivatives. However, it is not clear whether human umbilical cord blood plasma (UCBP), as the surplusage of umbilical cord blood mesenchymal stem cell extraction, could be used. In this study, in order to make the best of umbilical cord blood, the human UCBP was dialyzed to replace fetal bovine serum (FBS) in the culture medium. hUC-MSCs were cultured in the new medium. Cell growth rate, specific biomarkers, and differentiation properties were detected to characterize the cell proliferation and MSC-specific properties. The hUC-MSCs cultured in such derived medium were verified with proliferation rate, cluster differentiation markers, cell cycle, as well as differentiation capabilities. Such dialyzed human UCBP is fully comparable with, if not superior to, FBS in deriving and culturing hUC-MSCs.

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Acknowledgments

This study was funded by the National Natural Science Foundation of China (81070614), National Science and Technology Plan 973 of China (2009CB930201), and science and technical development project of Guangxi Chuang autonomous region (0993003A).

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Authors and Affiliations

  1. College of Life Science and Bioengineering, Beijing University of Technology, Beijing, 100124, China

    Yan Ding & Yi Zeng

  2. Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, China

    Yan Ding, Jing Bo Feng & Dong Sheng Li

  3. Reproductive Medical Center of Nanning Second People’s Hospital, Third Affiliated Hospital of Guangxi Medical University, Nanning, 530031, China

    Hua Yang & Ying Qiu

Authors
  1. Yan Ding
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  2. Hua Yang
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  3. Jing Bo Feng
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  4. Ying Qiu
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  5. Dong Sheng Li
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  6. Yi Zeng
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Corresponding authors

Correspondence to Dong Sheng Li or Yi Zeng.

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Editor: T. Okamoto

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Ding, Y., Yang, H., Feng, J.B. et al. Human umbilical cord-derived MSC culture: the replacement of animal sera with human cord blood plasma. In Vitro Cell.Dev.Biol.-Animal 49, 771–777 (2013). https://doi.org/10.1007/s11626-013-9663-8

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  • DOI: https://doi.org/10.1007/s11626-013-9663-8

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