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Influence of rocky substrata on three-dimensional sponge cells model development

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Abstract

Many marine and freshwater organisms are rocky bottom dwellers, and the mineralogical composition of the substratum is known to potentially condition their biology and ecology. In this work, we propose the use of 3D sponge cellular aggregates, called primmorphs, as suitable models for a multidisciplinary study of the mechanisms which regulate the biological responses triggered by the contact with different inorganic substrata. In our experiments, primmorphs obtained from the marine sponge Petrosia ficiformis (Poiret, 1789) were reared on calcium carbonate or on quartzitic substrata, respectively, and their morphological development was described. In parallel, the quantitative expression levels of two genes, silicatein and heat shock protein 70 (HSP70), were evaluated. The first gene is strictly correlated to spiculogenesis and sponge growth, while the second is an important indicator of stress. The results achieved with this in vitro model clearly demonstrate that quartzitic substrata determine the increase of silicatein gene expression, a lower expression of HSP70 gene, and a remarkable difference in primmorphs morphology compared to the analogous samples grown on marble.

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Acknowledgments

This work was partially supported by EC fund to MG, by Liguria Region, Area Marine Biotechnology Funds to MG, by Italian Research Ministry Fund (PRIN 2007) to MG, and by University of Genova funds to CC. We thank Laura Negretti for technical support in the SEM analysis of rocks. The experiments of this study comply with the current laws of Italy.

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Authors and Affiliations

  1. Centro Biotecnologie Avanzate, Largo Rosanna Benzi, 10, 16132, Genova, Italy

    Marina Pozzolini, Laura Valisano & Marco Giovine

  2. CEBR, Università degli Studi di Genova, Viale Benedetto XV, 7, 16132, Genova, Italy

    Marina Pozzolini & Marco Giovine

  3. DIPTERIS, Università degli Studi di Genova, Corso Europa 26, 16132, Genova, Italy

    Carlo Cerrano & Stefano Schiaparelli

  4. DIMES-Sez. Biochimica, Università degli Studi di Genova, Viale Benedetto XV, 16132, Genova, Italy

    Mattia Menta & Umberto Benatti

  5. Dipartimento di Scienze del Mare, Università Politecnica delle Marche, 60131, Ancona, Italy

    Giorgio Bavestrello

  6. Dipartimento di Biologia, Laboratorio di biologia molecolare, Università degli Studi di Genova, Via Pastore 3, 16132, Genova, Italy

    Marco Giovine

Authors
  1. Marina Pozzolini
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  2. Laura Valisano
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  3. Carlo Cerrano
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  4. Mattia Menta
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  5. Stefano Schiaparelli
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  6. Giorgio Bavestrello
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  7. Umberto Benatti
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  8. Marco Giovine
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Corresponding author

Correspondence to Marco Giovine.

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Editor: J. Denry Sato

Pozzolini and Valisano equally contributed to this work.

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Pozzolini, M., Valisano, L., Cerrano, C. et al. Influence of rocky substrata on three-dimensional sponge cells model development. In Vitro Cell.Dev.Biol.-Animal 46, 140–147 (2010). https://doi.org/10.1007/s11626-009-9253-y

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  • DOI: https://doi.org/10.1007/s11626-009-9253-y

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