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Cross talk between hedgehog and bone morphogenetic proteins occurs during cardiomyogenesis in P19 cells

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Abstract

Hedgehog (Hh) signaling plays a role in heart morphogenesis and can initiate cardiomyogenesis in P19 cells. To determine if Hh signaling is essential for P19 cell cardiomyogenesis, we determined which Hh factors are expressed and the effect of Hh signal transduction inhibitors. Here, we find that the Hh gene family and their downstream mediators are expressed during cardiomyogenesis but an active Hh signaling pathway is not essential. However, loss of Hh signaling resulted in a delay of BMP-4, GATA-4, Gli2, and Meox1 expression during cardiomyogenesis. By using Noggin-overexpressing P19 cells, we determined that Hh signaling was not active during Noggin-mediated inhibition of cardiomyogenesis. Thus, there is cross talk between the Hh and BMP signaling pathways and the Hh pathway appears important for timely cardiomyogenesis.

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Acknowledgments

P.G. was supported by a Canadian Institute of Health Doctoral Research Award. I.S.S. was supported by a Canadian Institute of Aging Investigator Award. This work was supported by grant MOP-53277 (to I. S. S.) from the Canadian Institutes of Health Research.

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Authors and Affiliations

  1. Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, 451 Smyth Road, Ottawa, Ontario, Canada, K1H 8M5

    Peter Junior Gianakopoulos & Ilona S. Skerjanc

  2. Department of Biochemistry, Medical Sciences Building, University of Western Ontario, London, Ontario, Canada, N6A 5C1

    Peter Junior Gianakopoulos

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  1. Peter Junior Gianakopoulos
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  2. Ilona S. Skerjanc
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Correspondence to Ilona S. Skerjanc.

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Editor: J. Denry Sato

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Gianakopoulos, P.J., Skerjanc, I.S. Cross talk between hedgehog and bone morphogenetic proteins occurs during cardiomyogenesis in P19 cells. In Vitro Cell.Dev.Biol.-Animal 45, 566–572 (2009). https://doi.org/10.1007/s11626-009-9228-z

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