Journal of Gastrointestinal Surgery

, Volume 18, Issue 2, pp 286–294

High-Fat Diet Enhances Villus Growth During the Adaptation Response to Massive Proximal Small Bowel Resection

  • Pamela M. Choi
  • Raphael C. Sun
  • Jun Guo
  • Christopher R. Erwin
  • Brad W. Warner
2013 SSAT Plenary Presentation

DOI: 10.1007/s11605-013-2338-7

Cite this article as:
Choi, P.M., Sun, R.C., Guo, J. et al. J Gastrointest Surg (2014) 18: 286. doi:10.1007/s11605-013-2338-7

Abstract

Previous studies have shown that high-fat diet (HFD) enhances adaptation if provided immediately following small bowel resection (SBR). The purpose of this study was to determine if HFD could further enhance villus growth after resection-induced adaptation had already taken place. C57/Bl6 mice underwent a 50 % proximal SBR or sham operation and were then provided a standard rodent liquid diet (LD) ad lib. After a typical period of adaptation (7 days), SBR and sham-operated mice were randomized to receive either LD or HFD (42 % kcal fat) for an additional 7 days. Mice were then harvested, and small intestine was collected for analysis. Adaptation occurred in both SBR groups; however, the SBR/HFD had significantly increased villus height compared to SBR/LD. Reverse transcription–polymerase chain reaction of villus enterocytes showed a marked increase in CD36 expression in the SBR/HFD group compared with SBR/LD mice. While exposure to increased enteral fat alone did not affect villus morphology in sham-operated mice, HFD significantly increased villus growth in the setting of resection-induced adaptation, supporting the clinical utility of enteral fat in augmenting adaptation. Increased expression of CD36 suggests a possible mechanistic role in dietary fat metabolism and villus growth in the setting of short gut syndrome.

Keywords

Short gut syndromeSmall bowel adaptationHigh-fat dietCD36

Copyright information

© The Society for Surgery of the Alimentary Tract 2013

Authors and Affiliations

  • Pamela M. Choi
    • 1
    • 2
  • Raphael C. Sun
    • 1
    • 2
  • Jun Guo
    • 1
    • 2
  • Christopher R. Erwin
    • 1
    • 2
  • Brad W. Warner
    • 1
    • 2
  1. 1.Division of Pediatric SurgerySt Louis Children’s HospitalSt. LouisUSA
  2. 2.Department of SurgeryWashington University School of MedicineSt. LouisUSA