Antioxidative protective effect of icariin on the FeSO4/H2O2-damaged human sperm based on confocal raman micro-spectroscopy

  • Zhan-sen Huang (黄展森)
  • Heng-jun Xiao (肖恒军)
  • Tao Qi (齐 涛)
  • Zhi-ming Hu (胡志明)
  • Hao Li (李 浩)
  • Di-ling Chen (陈地灵)
  • Ya-lin Xu (徐亚林)
  • Jun Chen (陈 俊)
Article

DOI: 10.1007/s11596-014-1348-3

Cite this article as:
Huang, Z., Xiao, H., Qi, T. et al. J. Huazhong Univ. Sci. Technol. [Med. Sci.] (2014) 34: 755. doi:10.1007/s11596-014-1348-3
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Summary

Oxidative stress is implicated in male infertility and significantly higher reactive oxygen species are detected in 25% of infertile males. Although different agents of various alternative medicines, including traditional Chinese medicine, have been tried with varying success, evidence remains limited on whether and how much herbs or supplements might help increase the anti-oxidant ability of the sperm. This study examined the anti-oxidative effects of icariin, a flavonoid isolated from HerbaEpimedii, on the human sperm. We prepared the FeSO4/H2O2-damaged human sperms, which were co-cultured with icariin in vitro, and then observed the changes of the sperm by employing Raman micro-spectroscopy. The results showed that Raman mapping with a 514 nm excitation laser allowed clear differentiation of the nucleus, neck, and, in particular, the mitochondria-rich middle piece of a human sperm cell. The effect of icariin on different organelles of the sperm was quantified by localized spectral Raman signatures obtained within milli-seconds, and icariin could keep the “Raman fingerprint” of the human sperm the same as the control groups, suggesting that icariin could protect the human sperm from being damaged by FeSO4/H2O2. Icariin may serve as a tonifying and replenishing agent of herbal origin for enhancing reproductive functions.

Key words

anti-oxidative protection mechanism icariin human sperm Raman spectroscopy 

Copyright information

© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Zhan-sen Huang (黄展森)
    • 1
  • Heng-jun Xiao (肖恒军)
    • 2
  • Tao Qi (齐 涛)
    • 1
  • Zhi-ming Hu (胡志明)
    • 1
  • Hao Li (李 浩)
    • 1
  • Di-ling Chen (陈地灵)
    • 3
  • Ya-lin Xu (徐亚林)
    • 4
  • Jun Chen (陈 俊)
    • 1
  1. 1.Departerment of Infertility and Sexual Medicinethe Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouChina
  2. 2.Department of Urologythe Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouChina
  3. 3.Guangdong Institute of MicrobiologyGuangzhouChina
  4. 4.Kaiping Central HospitalKaiping, GuangzhouChina

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