Journal of Huazhong University of Science and Technology [Medical Sciences]

, Volume 33, Issue 4, pp 530–535

Role of GSK-3β in isoflurane-induced neuroinflammation and cognitive dysfunction in aged rats

  • Shi-yong Li李世勇
  • Xin Chen陈 欣
  • Ye-ling Chen陈晔凌
  • Lei Tan谭 蕾
  • Yi-lin Zhao赵以林
  • Jin-tao Wang王金韬
  • Qiang Xiang向 强
  • Ai-lin Luo罗爱林
Article

DOI: 10.1007/s11596-013-1154-3

Cite this article as:
Li, S., Chen, X., Chen, Y. et al. J. Huazhong Univ. Sci. Technol. [Med. Sci.] (2013) 33: 530. doi:10.1007/s11596-013-1154-3

Summary

This study investigated the role of glycogen synthase kinase-3β (GSK-3β) in isoflurane-induced neuroinflammation and cognitive dysfunction in aged rats. The hippocampi were dissected from aged rats which had been intraperitoneally administered lithium chloride (LiCl, 100 mg/kg) and then exposed to 1.4% isoflurane for 6 h. The expression of GSK-3β was detected by Western blotting. The mRNA and protein expression levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 were measured by real-time PCR and enzyme-linked immunosorbent assay (ELISA), respectively. Morris water maze was employed to detect spatial memory ability of rats. The results revealed that the level of GSK-3β was upregulated after isofurane exposure. Real-time PCR analysis demonstrated that isoflurane anesthesia increased mRNA levels of TNF-α, IL-1β and IL-6, which was consistent with the ELISA results. However, these changes were reversed by prophylactic LiCl, a non-selective inhibitor of GSK-3β. Additionally, we discovered that LiCl alleviated isoflurane-induced cognitive impairment in aged rats. Furthermore, the role of GSK-3β in isoflurae-induced neuroinflammation and cognitive dysfunction was associated with acetylation of NF-κB p65 (Lys310). In conclusion, these results suggested that GSK-3β is associated with isoflurane-induced upregulation of proinflammatory cytokines and cognitive disorder in aged rats.

Key words

isofluraneGSK-3βcognitive dysfunctionneuroinflammation

Copyright information

© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Shi-yong Li李世勇
    • 1
  • Xin Chen陈 欣
    • 1
  • Ye-ling Chen陈晔凌
    • 1
  • Lei Tan谭 蕾
    • 1
  • Yi-lin Zhao赵以林
    • 1
  • Jin-tao Wang王金韬
    • 1
  • Qiang Xiang向 强
    • 2
  • Ai-lin Luo罗爱林
    • 1
  1. 1.Department of Anesthesiology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Anesthesiology, Wuhan Children’s Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina