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Establishment of reproducible xenotransplantation model of T cell acute lymphoblastic leukemia in NOD/SCID mice

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Summary

T cell acute lymphoblastic leukemia (T-ALL) is an aggressive leukemia. However the poor prognosis and low morbidity restrict further analysis of the disease. Therefore there is an increasing demand to develop animal models for identifying novel therapeutic approaches. In this study, we inoculated the anti-mouse CD122 monoclonal antibody conditioned NOD/SCID mice with the leukemia cells from 9 T-ALL patients and 1 cell line via the tail vein. Four of the 9 patients and the cell line were successfully engrafted. Flow cytometry detected high percentage of human CD45+ cells in recipient mice. Immunohistochemistry showed infiltration of human CD45+ cells in different organs. Serial transplantation was also achieved. In vivo drug treatment showed that dexamethasone could extend survival, which was consistent with clinical observation. These results demonstrated that we successfully established 5 xenotransplantation models of T-ALL in anti-mCD122 mAb conditioned NOD/SCID mice, which recapitulated the characteristics of original disease.

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Authors and Affiliations

  1. Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Di Wang  (王 迪), Na Wang  (王 娜), Zhe Geng  (耿 哲), Jianfeng Zhou  (周剑峰) & Liang Huang  (黄 亮)

  2. Crown Bioscience Inc. (Beijing), Beijing, 102200, China

    Yan Zhang  (张 艳), Shuyan Ma  (马淑燕) & Pengfei Zhou  (周鹏飞)

Authors
  1. Di Wang  (王 迪)
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  2. Na Wang  (王 娜)
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  3. Yan Zhang  (张 艳)
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  4. Shuyan Ma  (马淑燕)
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  5. Zhe Geng  (耿 哲)
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  6. Pengfei Zhou  (周鹏飞)
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  7. Jianfeng Zhou  (周剑峰)
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  8. Liang Huang  (黄 亮)
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Additional information

This project was supported in part by the National Natural Science Foundation of China (No. 81025011 and No. 81090414).

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Wang, D., Wang, N., Zhang, Y. et al. Establishment of reproducible xenotransplantation model of T cell acute lymphoblastic leukemia in NOD/SCID mice. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 32, 511–516 (2012). https://doi.org/10.1007/s11596-012-0088-5

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  • DOI: https://doi.org/10.1007/s11596-012-0088-5

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