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Role of RANTES and its receptor in gastric cancer metastasis

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Summary

This study examined the role of regulated upon activation normal T cell expressed and secreted (RANTES) and its receptor C-C chemokine receptor type 5 (CCR5) in gastric cancer metastasis and the associated mechanism. The expression of RANTES and CCR5 was detected by using immunohistochemical staining and Western blotting in the gastric cancer tissues obtained from 60 gastric cancer patients with or without lymph node metastasis (n=30 in each). The results showed that the expression levels of RANTES and CCR5 were higher in gastric cancer with lymph node metastasis than in that without metastasis (P<0.05). The expression levels of RANTES in 30 lymph nodes with cancerous invasion were higher than in 30 normal lymph nodes (P<0.05). Chemotactic test revealed that the number of migrating gastric cancer cells (n=295.0±54.6) induced by the protein of cancer-invading lymph nodes was greater than that by the protein mixture from cancer-invading lymph nodes and RANTES antibody (n=42.5±11.6) (P<0.05). RT-PCR showed that the expression levels of the main Th1 cytokines (IL-2, Γ-IFN) were lower in gastric cancer with lymph node metastasis (2.22±0.90, 3.26±1.15 respectively) than in that without metastasis (3.07±1.67, 4.77±1.52 respectively) (P<0.05), but the expression level of the main Th 2 cytokine (IL-10) was higher in gastric cancer with lymph nodes metastasis (6.06±2.04) than in that without metastasis (4.88±1.87) (P<0.05). It was concluded that RANTES and its receptor CCR5 may contribute to gastric cancer metastasis through influencing the balance of Th1/Th2. RANTES and CCR5 may become a marker of gastric cancer metastasis.

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Authors and Affiliations

  1. Gastrointestinal Surgery Center, Huazhong University of Science and Technology, Wuhan, 430030, China

    Zhixin Cao  (曹志新), Xiangshang Xu  (徐向上), Xuelai Luo  (罗学来), Xiaolan Li  (李小兰), Deding Tao  (陶德定), Junbo Hu  (胡俊波) & Jianping Gong  (龚建平)

  2. Cancer Biology Research Center, Huazhong University of Science and Technology, Wuhan, 430030, China

    Li Li  (李 莉)

  3. Out-patient Department of Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Bin Huang  (黄 彬)

Authors
  1. Zhixin Cao  (曹志新)
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  2. Xiangshang Xu  (徐向上)
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  3. Xuelai Luo  (罗学来)
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  4. Li Li  (李 莉)
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  5. Bin Huang  (黄 彬)
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  6. Xiaolan Li  (李小兰)
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  7. Deding Tao  (陶德定)
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  8. Junbo Hu  (胡俊波)
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  9. Jianping Gong  (龚建平)
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Corresponding author

Correspondence to Jianping Gong  (龚建平).

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These authors contributed equally to this work.

This work was supported by a grant from Natural Sciences Foundation of Hubei Province of China (No. 2006ABA098).

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Cao, Z., Xu, X., Luo, X. et al. Role of RANTES and its receptor in gastric cancer metastasis. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 31, 342–347 (2011). https://doi.org/10.1007/s11596-011-0378-3

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  • DOI: https://doi.org/10.1007/s11596-011-0378-3

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