Abstract
Schwann cells play a key role in peripheral nerve growth and regeneration. The aim of this study was to evaluate the effects of RGD peptides on Schwann cell behavior, and to identify the effects of the modified PDLLA films with RGD in vivo. The results revealed that RGD coating with the concentration of 100–500 ug/mL promoted the cell proliferation and boosted the cell migration. Molecularly, RGD coating also enhanced the expression of the proliferation related genes (c-fos and c-jun) and the cell behavior related genes (actin, tublin, tau and MAP1) at first stages of the seeding, which is similar to the effects from laminin coating. In vivo, RGD addition improved the recovery efficiency of the transected nerve in regard of the more survived Schwann cells in vivo and the formation of more mature myelin sheath. Taken together, RGD peptides are good candidates to enhance the biocompatibility of the biomaterials and facilitate the peripheral nerve regeneration by prompting responses in Schwann cells.
References
Epple M, Rueger J M Festk. Orperchemie Und Chirurgie [J]. Nachr. Chem. Tech. Lab., 1999, 47: 1 405–1 410
Rasmussen J R, Stedronsky E R, Whitesides G M. Introduction, Modification, and Characterization of Functional Groups on the Surface of Low-density Polyethylene Film [J]. J. Am. Chem. Soc., 1977, 99: 4 736–4 745
Lee J H, Jung J W, Kang I K, et al. Cell Behaviour on Polymer Surfaces with Different Functional Groups [J]. Biomaterials, 1994, 15: 705–711
Brandley B K, Schnaar R L. Covalent Attachment of an ARG-GLY-ASP Sequence Peptide to Derivatizable Polyacrylamide Surfaces: Support of Fibroblast Adhesion and Long-term Growth [J]. Anal. Biochem., 1998, 172: 270–278
Lin H B, Zhao Z C, Garcia-Echeverria C, et al. Synthesis of a Novel Polyurethane Copolymer Containing Covalently AttachedRGD Peptide [J]. J Biomater. Sci., 1991, 3: 217–227
Falb R D, Grode G A. Covalent Bonding of Proteins to Solid Surfaces [J]. Fed. Proc., 1971, 30: 1 688–1 691
Kobayashi K, Sumitomo H. Oligosaccharide-carrying Styrenetype Macromers. Polymerization Andspecific Interactions between the Polymers and Liver Cells [J]. J. Macromol. Sci. Chem., 1988, 25: 655–667
Weigel P H, Schnaar R L, Kuhlenschmidt M S, et al. Adhesion of Hepatocytes to Immobilized Sugars: A Threshold Phenomenon [J]. J. Biol. Chem., 1979, 354: 10 830–10 838
Ruoslahti E, Pierschbacher M D. New Perspectives in Cell Adhesion: RGD and Integrins [J]. Science, 1987, 238: 491–497
Albelda S M, Buck C A. Integrins and Other Cell Adhesion Molecules [J]. FASEB. J., 1990, 4: 2 868–2 880
Travis J. Biotech Gets a Grip on Cell Adhesion [J]. Science, 1993, 260: 906–908
Kammerer P W, Heller M, Brieger J, et al. Immobilisation of Linear and Cyclic RGD-peptides on Titanium Surfaces and Their Impact on Endothelial Cell Adhesion and Proliferation [J]. Eur. Cells. Mater., 2011, 21: 364–372
Shu X Z, Ghosh K, Liu Y, et al. Attachment and Spreading of Fibroblasts on an RGD Peptide-modified Injectable Hyaluronan Hydrogel [J]. J. Biomed. Mater. Res. A, 2004, 68: 365–375
Li B, Chen J X, Wang J H C. RGD Peptide-conjugated poly (dimethylsiloxane) Promotes Adhesion, Proliferation, and Collagen Secretion of Human Fibroblasts [J]. J. Biomed. Mater. Res. A, 2006, 79: 989–998
Hersel U, Dahmen C, Kessler H. RGD Modified Polymers: Biomaterials for Stimulated Cell Adhesion and Beyond [J]. Biomaterials, 2003, 24: 4 385–4 415
Davis D H, Giannoulis C S, Johnsonb R W, et al. Immobilization of RGD to Silicon Surfaces for Enhanced Cell Adhesion and Proliferation[J]. Biomaterials, 2002, 23: 4 019–4 027
Kafi M A, El-Said W A, Kim T H, et al. Cell Adhesion, Spreading, and Proliferation on Surface Functionalized with RGD Nanopillar Arrays [J]. Biomaterials, 2012, 33: 731–739
Puleo D A, Bizios R. RGDS Tetrapeptide Binds to Osteoblasts and Inhibits Fibronectin-mediated Adhesion [J]. Bone, 1991, 12:271–276
Rezania A, Thomas C H, Branger A B, et al. The Detachment Strength and Morphology of Bone Cells Contacting Materials Modified with a Peptide Sequence Found within Bone Sialoprotein [J]. J. Biomed. Mater. Res., 1997, 37:9–19
Massia S, Hubbell J. Covalent Surface Immobilization of Arg-Gly-Asp- and Tyr-Ile-Gly-Ser-Arg-containing Peptides to Obtain Well-defined Cell-adhesive Substrates [J]. Anal. Biochem., 1990, 187: 292–301
Qiongjiao Y, Yixia Y, Binbin L. Use New PLGL-RGD-NGF Nerve Conduits for Promoting Peripheral Nerve Regeneration [J]. Biomedical Engineering Online, 2012, 11: 36–40
Wohlrab S, Müller S, Schmidt A, et al. Cell Adhesion and Proliferation on RGD-modified Recombinant Spider Silk Proteins [J]. Biomaterials, 2012, 33: 6 650–6 659
Wacker B K, Alford S K, Scott E A, et al. Endothelial Cell Migration on RGD-peptide-containing PEG Hydrogels in the Presence of Sphingosine 1-Phosphate [J]. Biophysical J., 2008, 94: 273–285
Zayzafoon M, Stell C, Irwin R, et al. Extracellular Glucose Influences Osteoblast Differentiation and c-jun Expression [J]. J. Cell Bioche., 2000, 79:301–310
Li H H, He B, Peng H, et al. Effects of Pyrroloquinoline Quinone on Proliferation and Expression of c-fos, c-jun, CREB and PCNA in Cultured Schwann Cells [J]. Zhonghua Zheng Xing Wai Ke Za Zhi, 2011, 27: 298–303
Peris L, Thery M, Faure J, et al. Tubulin Tyrosination is a Major Factor Affecting the Recruitment of CAP-Gly Proteins at Microtubule Plus Ends[J]. J. Cell Biol., 2006, 174: 839–849
Ramey V H, Wang H W, Nakajima Y, et al. The Dam1 Ring Binds to the E-hook of Tubulin and Diffuses Along the Microtubule[J]. Mol. Biol. Cell, 2011, 22: 457–466
Trinczek B, Ebneth A, Mandelkow E M, et al. Tau Regulates the Attachment/Detachment but not the Speed of Motors in Microtubule-Dependent Transport of Single Vesicles and Organelles [J]. J. Cell. Sci., 1999, 112: 2 355–2 367
Fuhrmann-Stroissnigg H, Noiges R, Descovich L, et al. The Light Chains of Microtubule-associated Proteins MAP1A and MAP1B Interact with α1-syntrophin in the Central and Peripheral Nervous System [J]. PLos One, 2012, 7: 49 722–49 727
Liu W Q, Martinez J A, Durand J, et al. RGD-mediated Adhesive Interactions are Important for Peripheral Axon Outgrowth in Vivo [J]. Neurobiol. Dis., 2009, 34:11–22
Afshari F T, Kwok J C, White L. Schwann Cell Migration Is Integrin-Dependent and Inhibited by Astrocyte-produced Aggrecan [J]. Glia, 2010, 58: 857–869
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Fang, X., Qiu, T., Xie, L. et al. RGD gifted PDLLA-PRGD conduits promotes the sciatic nerve regeneration. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 29, 620–625 (2014). https://doi.org/10.1007/s11595-014-0968-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11595-014-0968-6