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Diversity of endophytic fungi of common yew (Taxus baccata L.) in Iran

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Abstract

To characterize the endophytic fungi of common yew, 125 fungal strains were isolated from 80 healthy twig and bark samples. Of them, 41 (32.8%) isolates were identified on the basis of morphological features and 84 (67.2%) isolates were sterile. Of the 41 morphotypes, 40 isolates belonged to 21 genera and 26 species, with an isolate as anamorphic Xylaria. Most of the identified endophytic fungi belonged to mycelia sterilia (67.2%) and Ascomycota (32%) and the others belonged to Zygomycota (0.8%). Cladosporium and Alternaria were the dominant genera. Most of the identified species are new endophytes for common yew and Pseudodictyosporium elegans, Cladosporium basi-inflatum, C. perangustum, C. subtilissimum, Geniculosporium serpens, Phoma pratorum, Paraphoma fimeti, Phomopsis archeri, Sclerostagonospora cycadis, and Seimatosporium cf. pezizoides are reported as new taxa for the mycoflora of Iran. ITS rDNA phylogeny of 26 selected isolates revealed four clades, 1, 2, 3, and 4, corresponding to Sordariomycetes, Dothideomycetes, Eurotiomycetes, and Dothideomycetes, respectively. Clade 1 included four groups, A, B, C, and D, representing, respectively, the Xylariales, Trichosphaeriales, Hypocreales, and Diaporthales identified. The results have shown that T. baccata in Iran harbors a wide and significant diversity of fungal endophytes.

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Acknowledgments

The present research has been done based on a grant (73145563/6/05) given by the University of Tehran. So, the authors are pleased to appreciate the University of Tehran.

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  1. Department of Plant Protection, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, 31587-77871, Iran

    Saeideh Jam Ashkezari & Khalil-Berdi Fotouhifar

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  1. Saeideh Jam Ashkezari
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  2. Khalil-Berdi Fotouhifar
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Correspondence to Khalil-Berdi Fotouhifar.

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Jam Ashkezari, S., Fotouhifar, KB. Diversity of endophytic fungi of common yew (Taxus baccata L.) in Iran. Mycol Progress 16, 247–256 (2017). https://doi.org/10.1007/s11557-017-1274-4

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