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Six newly developed microsatellite markers of Laccaria amethystina, using an improved CSSR approach

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Abstract

Microsatellite markers were established by an improved combined simple sequence repeat (CSSR) approach for the ectomycorrhizal basidiomycete Laccaria amethystina. Six markers delivered codominant polymorphic results, with up to six different alleles. They were tested with DNA originating from sporocarps and ectomycorrhizal root tips from silver fir (Abies alba), collected in the northern Black Forest. Sporocarps and ectoymcorrhizae exhibited similar allelic profiles, without any distorting influence of the host tree species. Results were compared to allelic profiles delivered by three published Asian markers. Allelic frequencies of the new markers showed a higher resolution of individuals within the population community, but partially a lower grade of heterozygosity. Possible reasons are discussed, and further questions addressed.

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References

  • Agerer R (1987–98) Colour Atlas of Ectomycorrhizae. 1st-11th delivery. Einhorn, Schwäbisch-Gmünd

    Google Scholar 

  • Baar J, Ozinga WA, Kuyper TW (1994) Spatial distribution of Laccaria bicolor genets reflected by sporocarps after removal of litter and humus layers in a Pinus sylvestris forest. Mycol Res 98:726–728

    Google Scholar 

  • Bergemann SE, Miller SL (2002) Size, distribution, and persistence of genets in local populations of the late-stage ectomycorrhizal basidiomycete, Russula brevipes. New Phytol 156:313–320

    Article  CAS  Google Scholar 

  • Bonello P, Bruns TD, Gardes M (1998) Genetic structure of a natural population of the ectomycorrhizal fungus Suillus pungens. New Phytol 138:533–542

    Article  CAS  Google Scholar 

  • Dahlberg A, Stenlid J (1990) Population structure and dynamics in Suillus bovinus as indicated by spatial distribution of fungal clones. New Phytol 115:487–493

    Article  Google Scholar 

  • Dahlberg A, Stenlid J (1994) Size, distribution and biomass of genets in populations of Suillus bovinus (L. Fr.) Roussel revealed by somatic incompatibility. New Phytol 128:225–234

    Article  Google Scholar 

  • De la Bastide PY, Kropp BR, Piché Y (1994) Spatial distribution and temporal persistence of discrete genotypes of the ectomycorrhizal fungus Laccaria bicolor (Maire) Orton. New Phytol 127:547–556

    Article  Google Scholar 

  • Dumolin S, Demesure B, Petit RJ (1995) Inheritance of chloroplast and mitochondrial genomes in pedunculate oak investigated with an efficient PCR method. Theor Appl Genet 91:1253–1256

    Article  CAS  Google Scholar 

  • Fiore-Donno A-M, Martin F (2001) Populations of ectomycorrhizal Laccaria amethystina and Xerocomus spp. show contrasting colonization patterns in a mixed forest. New Phytol 152:533–542

    Article  CAS  Google Scholar 

  • Gherbi H, Delaruelle C, Selosse M-A, Martin F (1999) High genetic diversity in a population of the ectomycorrhizal basidiomycete Laccaria amethystina in a 150-year-old beech forest. Mol Ecol 8:2003–2013

    Article  PubMed  CAS  Google Scholar 

  • Gryta H, Debaud J-C, Effosse A, Gay G, Marmeisse R (1997) Fine-scale structure of populations of the ectomycorrhizal fungus Hebeloma cylindrosporum in coastal sand dune forest ecosystems. Mol Ecol 6:353–364

    Article  Google Scholar 

  • Gryta H, Carriconde F, Charcosset JY, Jargeat P, Gardes M (2006) Population dynamics of the ectomycorrhizal fungal species Tricholoma populinum and Tricholoma scalpturatum associated with black poplar under differing environmental conditions. Environ Microbiol 8:773–786

    Article  PubMed  CAS  Google Scholar 

  • Hayden MJ, Stephenson P, Logojan AM, Khatkar D, Rogers C, Koebner RMD, Snape JW, Sharp PJ (2004) A new approach to extending the wheat marker pool by anchored PCR amplification of compound SSRs. Theor Appl Genet 108:733–742

    Article  PubMed  CAS  Google Scholar 

  • Horton TR, Bruns TD (1998) Multiple-host fungi are the most frequent and abundant ectomycorrhizal types in a mixed stand of Douglas fir (Pseudotsuga menziesii) and bishop pine (Pinus muricata). New Phytol 139:331–339

    Article  Google Scholar 

  • Jacobson KM, Miller OK Jr, Turner BJ (1993) Randomly amplified polymorphic DNA markers are superior to somatic incompatibility tests for discriminating genotypes in natural populations of the ectomycorrhizal fungus Suillus granulatus. Proc Natl Acad Sci USA 90:9159–9163

    Article  PubMed  CAS  Google Scholar 

  • Jany J-L, Bousquet J, Gagné A, Khasa DP (2005) Simple sequence repeat (SSR) markers in the ectomycorrhizal fungus Laccaria bicolor for environmental monitoring of introduced strains and molecular ecology applications. Brit Mycol Soc (Elsevier Ltd. doi:10.1016/j.mycres.2005.09.011)

  • Kjøller R (2006) Disproportionate abundance between ectomycorrhizal root tips and their associated mycelia. FEMS Microbiol Ecol 58(2):214–224

    Article  PubMed  CAS  Google Scholar 

  • Kretzer AM, Molin R, Spatafora JW (2000) Microsatellite markers for the ectonmycorrhizal basidiomycete Rhizopogon vinicolor. Mol Ecol 9:1190–1191

    Article  PubMed  CAS  Google Scholar 

  • Kretzer AM, Dunham S, Molina R, Spatafora JW (2003) Microsatellite markers reveal the below ground distribution of genets in two species of Rhizopogon forming tuberculate ectomycorrhizas on Douglas fir. New Phytol 161:313–320

    Article  CAS  Google Scholar 

  • Lian CL, Zhou Z, Hogetsu T (2001) A simple method for developing microsatellite markers using amplified fragments of inter-simple sequence repeat (ISSR). J Plant Res 114:381–385

    Article  CAS  Google Scholar 

  • Lian CL Narimatsu M, Nara K, Hogets T (2006a) Tricholoma matsutake in a natural Pinus densiflora forest: correspondence between above- and below-ground genets, association with multiple host trees and alteration of existing ectomycorrhizal communities. New Phytol 171:825–836

    Article  PubMed  Google Scholar 

  • Lian CL, Wadud MA, Geng Q, Shimatani K, Hogetsu T (2006b) An improved technique for isolating codominant compound microsatellite markers. J Plant Res 119:415–417

    Article  PubMed  CAS  Google Scholar 

  • Lim S, Notley-McRobb L, Lim M, Carter DA (2004) A comparison of the nature and abundance of microsatellites in 14 fungal genomes. Fung Genet Biol 41:1025–1036

    Article  CAS  Google Scholar 

  • Martin F, Selosse M-A, Le Tacon F (1999) The nuclear rDNA intergenic spacer of the ectomycorrhizal basidiomycete Laccaria bicolor: structural analysis and allelic polymorphism. Microbiology 145:1605–1611

    Article  PubMed  CAS  Google Scholar 

  • Mueller GM (1992) Systematics of Laccaria (Agaricales) in the Continental United States and Canada, with discussion on extralimital taxa and descriptions of extant types. Fieldiana Bot New Ser 30:1–158

    Google Scholar 

  • Nara K (2006) Ectomycorrhizal networks and seedling establishment during early primary succession. New Phytol 169(1):169–178

    Article  PubMed  CAS  Google Scholar 

  • Peakall R, Smouse PE (2005) GENALEX 6: Genetic Analysis in Excel. Population Genetic Software for Teaching and Research. Australian national University, Canberra, Australia. http://www.anu.edu.au/BoZo/GenAlEx/

  • Péréz-Hérnandez JB, Swennen R, Sagi L (2006) Number and accuracy of T-DNA insertions in transgenic Banana (Musa spp.) plants characterized by an improved anchored PCR technique. Transgen Res 15:139–150

    Article  CAS  Google Scholar 

  • Rassmann K, Schlötterer C, Tautz D (1991) Isolation of simple-sequence loci for use in polymerase chain reaction-based DNA fingerprinting. Electrophoresis 12:113–118

    Article  PubMed  CAS  Google Scholar 

  • Redecker D, Szaro TM, Bowman RJ, Bruns TD (2001) Small genets of Lactarius xanthogalactus, Russula cremicolor and Amanita francheti in late-stage ectomycorrhizal succession. Mol Ecol 10:1025–1034

    Article  PubMed  CAS  Google Scholar 

  • Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365–386

    PubMed  CAS  Google Scholar 

  • Sambrook JF, Maniatis T (1989) Molecular cloning: a laboratory manual: 1. Cold Spring Harbor Laboratory Press.

  • Wadud MA, Lian CL, Nara K, Ishida TA, Hogetsu T (2006) Development of microsatellite markers from an ectomycorrhizal fungus, Laccaria amethystina, by a dual-suppression-PCR technique. Mol Ecol Notes 6:130–132

    Article  CAS  Google Scholar 

  • Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16

    Article  PubMed  CAS  Google Scholar 

  • Zhou Z, Miwa M, Hogetsu T (1999) Analysis of genetic structure of a Suillus grevillei population in a Larix kaempferi stand by polymorphism of inter-simple sequence repeat (ISSR). New Phytol 144:55–63

    Article  CAS  Google Scholar 

  • Zhou Z, Miwa M, Matsuda Y, Hogetsu T (2001) Spatial distribution of the subterranenan mycelia and ectomycorrhizae of Suillus grevillei genets. J Plant Res 114:179–185

    Article  Google Scholar 

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Acknowledgements

We thank Dr. G.G. Vendramin (CNR, Florence, Italy) and Dr. Francis Martin (INRA, Nancy, France) with all their colleagues for introduction to microsatellite processing, the “mobility support” of EVOLTREE (EU-funded Network of excellence), the working group of Prof. Ziegenhagen (Conservation Biology, University of Marburg, Germany) for experience and support, the Forest directories and staff of the districts Freudenstadt and Calw (Baden-Württemberg, Germany) for friendly support and consultation, Dr. S. Liepelt (Conservation Biology, University of Marburg, Germany) for advice in handling the MegaBACE, C. Scherer for support in laboratory, and G. Braun and P. Touzani for administrative and collateral aid. The Project is funded by the DFG ([ZI 698/5] Deutsche Forschungsgemeinschaft, Bonn, Germany).

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Authors and Affiliations

  1. Faculty of Biology, Mycology, Philipps-University of Marburg, Karl-von-Frisch-Str. 8, 35032, Marburg, Germany

    Kathrin Donges, Dirk Schlobinski, Karl-Heinz Rexer & Gerhard Kost

  2. Bayerisches Amt für forstliche Saat- und Pflanzenzucht (ASP), Forstamtsplatz 1, 83317, Teisendorf, Germany

    Eva Cremer

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  1. Kathrin Donges
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  2. Dirk Schlobinski
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  3. Eva Cremer
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  4. Karl-Heinz Rexer
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  5. Gerhard Kost
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Correspondence to Kathrin Donges.

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Donges, K., Schlobinski, D., Cremer, E. et al. Six newly developed microsatellite markers of Laccaria amethystina, using an improved CSSR approach. Mycol Progress 7, 285–290 (2008). https://doi.org/10.1007/s11557-008-0564-2

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  • DOI: https://doi.org/10.1007/s11557-008-0564-2

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