Abstract
In recent years, new technologies have become available for imaging small animals. The use of animal models in basic and preclinical sciences, for example, offers the possibility of testing diagnostic markers and drugs, which is becoming crucial in the success and timeliness of research and is allowing a more efficient approach in defining study objectives and providing many advantages for both clinical research and the pharmaceutical industry. The use of these instruments offers data that are more predictive of the distribution and efficacy of a compound. The mouse, in particular, has become a key animal model system for studying human disease. It offers the possibility of manipulating its genome and producing accurate models for many human disorders, thus resulting in significant progress in understanding pathologenic mechanisms. In neurobiology, the possibility of simulating neurodegenerative diseases has enabled the development and validation of new treatment strategies based on gene therapy or cell grafting. Noninvasive imaging in small living animal models has gained increasing importance in preclinical research, itself becoming an independent specialty. The aim of this article is to review the characteristics of these systems and illustrate their main applications.
Riassunto
Negli ultimi anni si sono rese disponibili nuove apparecchiature per lo studio di piccoli animali. L’utilizzo di modelli sperimentali nelle scienze di base e pre-cliniche consente di verificare test diagnostici e farmaci, divenendo essenziale per il successo e la tempestività della ricerca, offrendo un approccio più efficace nella definizione degli obiettivi da studiare e notevoli vantaggi sia per la ricerca clinica sia per le industrie farmaceutiche. L’utilizzo di tali tecnologie consente di ottenere informazioni più predittive riguardo alla distribuzione o all’efficacia di una molecola. Il topo, in particolare, è un modello animale insostituibile per lo studio delle malattie umane. Esso offre la possibilità di manipolare il suo genoma e di riprodurre accuratamente malattie umane, consentendo progressi significativi nella comprensione dei meccanismi patogenetici. In neurobiologia, la possibilità di ricreare malattie neurodegenerative ha permesso lo sviluppo e la convalida di nuove strategie terapeutiche basate sulla terapia genica o sul trapianto di cellule. L’imaging non invasivo su piccoli animali in vivo ha acquisito un ruolo sempre maggiore nella ricerca pre-clinica fino a divenire un settore autonomo. Scopo di questo contributo è presentare le caratteristiche di queste apparecchiature illustrandone le principali applicazioni.
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Grassi, R., Cavaliere, C., Cozzolino, S. et al. Small animal imaging facility: new perspectives for the radiologist. Radiol med 114, 152–167 (2009). https://doi.org/10.1007/s11547-008-0352-8
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DOI: https://doi.org/10.1007/s11547-008-0352-8