Abstract
Crystals of calcium oxalate have been observed among members from most taxonomic groups of photosynthetic organisms ranging from the smallest algae to the largest trees. The biological roles for calcium oxalate crystal formation in plant growth and development include high-capacity calcium regulation, protection against herbivory, and tolerance to heavy metals. Using a variety of experimental approaches researchers have begun to unravel the complex mechanisms controlling formation of this biomineral. Given the important roles for calcium oxalate formation in plant survival and the antinutrient and pathological impact on human health through its presence in plant foods, researchers are avidly seeking a more comprehensive understanding of how these crystals form. Such an understanding will be useful in efforts to design strategies aimed at improving the nutritional quality and production of plant foods.
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References
Ahmed A K, Johnson K A (2000). The effect of the ammonium: nitrate nitrogen ration, total nitrogen, salinity (NaCl) and calcium on oxalate levels of Tetragonia tetragonioides Pallas. Kunz. J Hortic Sci Biotechnol, 75: 533–538
Arnott H J, Pautard F G E (1970). Calcification in plants. In: Biological Calcification: Cellular and Molecular Aspects (Schraer H, Ed.). New York: Appleton-Century-Crofts, 375–446
Assailly A (1954). Sur les rapports de l’oxalate de chaux et de l’amidon. Cr Acad Sci D, 238: 1902–1904
Barnabas A D, Arnott H J (1990). Calcium oxalate crystal formation in the bean (Phaseolus vulgaris L.) seed coat. Bot Gaz, 151(3): 331–341
Borchert R (1985). Calcium-induced patterns of calcium-oxalate crystals in isolated leaflets of Gleditsia triacanthos L. and Albizia julibrissin Durazz. Planta, 165(3): 301–310
Borchert R (1986). Calcium acetate induces calcium uptake and formation of calcium-oxalate crystals in isolated leaflets of Gleditsia tracanthos L. Planta, 168(4): 571–578
Bouropoulos N, Weiner S, Addadi L (2001). Calcium oxalate crystals in tomato and tobacco plants: morphology and in vitro interactions of crystal-associated macromolecules. Chemistry, 7(9): 1881–1888
Calmes J (1969). Contribution a l’etude du metabolisme de l’acide oxalique chez la Vigne vierge (Parthenocissus tricuspidata Planchon). Cr Acad Sci D, 269(6): 704–707
Calmes J, Carles J (1970). La repartition et l’evolution des cristaux d’oxalate de calcium dans les tissus de vigne vierge au cours d’un cycle de vegetation. B Soc Bot Fr, 117(5/6): 189–198
Catherwood D J, Savage G P, Mason S M, Scheffer J J C, Douglas J A (2007). Oxalate content of cormels of Japanese taro (Colocasia esculenta (L.) Schott) and the effect of cooking. J Food Compost Anal, 20(3–4): 147–151
Choi Y E, Harada E, Wada M, Tsuboi H, Morita Y, Kusano T, Sano H (2001). Detoxification of cadmium in tobacco plants: formation and active excretion of crystals containing cadmium and calcium through trichomes. Planta, 213(1): 45–50
Coté G G (2009). Diversity and distribution of idioblasts producing calcium oxalate crystals in Dieffenbachia seguine (Araceae). Am J Bot, 96(7): 1245–1254
Crofts A J, Leborgne-Castel N, Hillmer S, Robinson D G, Phillipson B, Carlsson L E, Ashford D A, Denecke J (1999). Saturation of the endoplasmic reticulum retention machinery reveals anterograde bulk flow. Plant Cell, 11(11): 2233–2248
De Yoreo J J, Qiu S R, Hoyer J R (2006). Molecular modulation of calcium oxalate crystallization. Am J Physiol Renal Physiol, 291(6): F1123–F1132
Franceschi V R (1989). Calcium oxalate formation is a rapid and reversible process in Lemna minor L. Protoplasma, 148(2–3): 130–137
Franceschi V R, Horner H T Jr (1979). Use of Psychotria puncata callus in study of calcium oxalate crystal idioblast formation. Z Pflanzenphysiol, 67: 61–75
Franceschi V R, Horner H T Jr (1980). Calcium oxalate crystals in plants. Bot Rev, 46(4): 361–427
Franceschi V R, Li X, Zhang D, Okita T W (1993). Calsequestrinlike calcium-binding protein is expressed in calcium-accumulating cells of Pistia stratiotes. Proc Natl Acad Sci USA, 90(15): 6986–6990
Franceschi V R, Loewus F A (1995). Oxalate biosynthesis and function in plants and fungi. In: Calcium Oxalate in Biological Systems (Khan S R Ed.). Boca Raton: CRC Press, 113–130
Franceschi V R, Nakata PA (2005). Calcium oxalate in plants: formation and function. Annu Rev Plant Biol, 56(1): 41–71
Franceschi V R, Schueren A M (1986). Incorporation of strontium into plant calcium oxalate crystals. Protoplasma, 130(2–3): 199–205
Franceschi V R, Tarlyn N M (2002). L-Ascorbic acid is accumulated in source leaf phloem and transported to sink tissues in plants. Plant Physiol, 130(2): 649–656
Frank E, Jensen WA (1970). On the formation of the pattern of crystal idiobalsts in Canavalia ensiformis DC. IV. The fine structure of the crystal cells. Planta, 95: 202–217
Frey-Wyssling A (1981). Crystallography of the two hydrates of crystalline calcium oxalate in plants. Am J Bot, 68(1): 130–141
Furuhashi T, Schwarzinger C, Miksik I, Smrz M, Beran A (2009). Molluscan shell evolution with review of shell calcification hypothesis. Comp Biochem Physiol B Biochem Mol Biol, 154(3): 351–371
Gallaher R N (1975). The occurrence of calcium in plant tissue as crystals of calcium oxalate. Commun Soil Sci Plan, 6(3): 315–330
Gélinas B, Seguin P (2007). Oxalate in grain amaranth. J Agric Food Chem, 55(12): 4789–4794
Green M A, Fry S C (2005). Vitamin C degradation in plant cells via enzymatic hydrolysis of 4-O-oxalyl-L-threonate. Nature, 433(7021): 83–87
Guo Z, Tan H, Zhu Z, Lu S, Zhou B (2005). Effect of intermediates on ascorbic acid and oxalate biosynthesis of rice and in relation to its stress resistance. Plant Physiol Biochem, 43(10–11): 955–962
Hartl W P, Klapper H, Barbier B, Ensikat H J, Dronskowski R, Müller P, Ostendorp G, Tye A, Bauer R, Barthlott W (2007). Diversity of calcium oxalate crystals in Cactaceae. Can J Bot, 85(5): 501–517
Heaney R P, Recker R R, Hinders S M (1988). Variability of calcium absorption. Am J Clin Nutr, 47(2): 262–264
Heaney R P, Weaver C M (1989). Oxalate: effect on calcium absorbability. Am J Clin Nutr, 50(4): 830–832
Heaney R P, Weaver C M (1990). Calcium absorption from kale. Am J Clin Nutr, 51(4): 656–657
Hodgkinson A (1977). Oxalic Acid Biology and Medicine. Academic Press: New York
Holmes R P, Goodman H O, Assimos D G (1995). Dietary oxalate and its intestinal absorption. Scanning Microsc, 9(4): 1109–1118, discussion 1118–1120
Holmes R P, Goodman H O, Assimos D G (2001). Contribution of dietary oxalate to urinary oxalate excretion. Kidney Int, 59(1): 270–276
Horner H T, Kausch A P, Wagner B L (2000). Ascorbic Acid: A precursor of oxalate in crystal idioblasts of Yucca Torreyi in liquid root culture. Int J Plant Sci, 161(6): 861–868
Horner H T, Wagner B L (1980). The association of druse crystals with the developing stomium of Capsicum annuum (Solanaceae) anthers. Am J Bot, 67(9): 1347–1360
Horner H T, Wagner B L (1995). Calcium oxalate formation in higher plants. In: Calcium Oxalate in Biological Systems. (Khan S R Ed.). Boca Raton: CRC Press, Florida, 53–72
Hudgins J W, Krekling T, Franceschi V R (2003). Distribution of calcium oxalate crystals in the secondary phloem of conifers: a constitutive defense mechanism? New Phytol, 159(3): 677–690
Ilarslan H, Palmer R G, Horner H T (2001). Calcium oxalate crystals in developing seeds of soybean. Ann Bot (Lond), 88(2): 243–257
Ji XM, Peng X X (2005). Oxalate accumulation as regulated by nitrogen forms and its relationship to photosynthesis in rice (Oryza sativa L.). J Int Plant Biol, 47(7): 831–838
Jou Y, Wang Y, Yen H E (2007). Vacuolar acidity, protein profile, and crystal composition of epidermal bladder cells of the halophyte Mesembryanthemum crystallinum. Funct Plant Biol, 34(4): 353–359
Katayama H, Fujibayashi Y, Nagaoka S, Sugimura Y (2007). Cell wall sheath surrounding calcium oxalate crystals in mulberry idioblasts. Protoplasma, 231(3–4): 245–248
Kausch A P, Horner H T (1984). Differentiation of raphide crystal idioblasts in isolated root cultures of Yucca torreyi (Agavaceae). Can J Bot, 62(7): 1474–1484
Kausch A P, Horner H T (1985). Absence of CeCl3-detectable peroxisomal glycolate-oxidase activity in developing raphide crystal idioblasts in leaves of Psychotria punctata Vatke and roots of Yucca torreyi L. Planta, 164(1): 35–43
Keates S E, Tarlyn N M, Loewus F A, Franceschi V R (2000). LAscorbic acid and L-galactose are sources for oxalic acid and calcium oxalate in Pistia stratiotes. Phytochemistry, 53(4): 433–440
Kochian L V (1995). Cellular mechanisms of aluminum toxicity and resistance in plants. Annu Rev Plant Physiol Plant Mol Biol, 46(1): 237–260
Korth K L, Doege S J, Park S H, Goggin F L, Wang Q, Gomez S K, Liu G, Jia L, Nakata P A (2006). Medicago truncatula mutants demonstrate the role of plant calcium oxalate crystals as an effective defense against chewing insects. Plant Physiol, 141(1): 188–195
Kostman T A, Franceschi V R (2000). Cell and calcium oxalate crystal growth is coordinated to achieve high-capacity calcium regulation in plants. Protoplasma, 214(3–4): 166–179
Kostman T A, Franceschi V R, Nakata P A (2003). Endoplasmic reticulum sub-compartments are involved in calcium sequestration within raphide crystal idioblasts of Pistia stratiotes L. Plant Sci, 165(1): 205–212
Kostman T A, Koscher J R (2003). L-galactono-gamma-lactone dehydrogenase is present in calcium oxalate crystal idioblasts of two plant species. Plant Physiol Biochem, 41(3): 201–206
Kostman T A, Tarlyn N M, Franceschi V R (2007). Autoradiography utilising labelled ascorbic acid reveals biochemical and morphological details in diverse calcium oxalate crystal-forming species. Funct Plant Biol, 34(4): 339–342
Kostman T A, Tarlyn N M, Loewus F A, Franceschi V R (2001). Biosynthesis of L-ascorbic acid and conversion of carbons 1 and 2 of L-ascorbic acid to oxalic acid occurs within individual calcium oxalate crystal idioblasts. Plant Physiol, 125(2): 634–640
Kröger N, Poulsen N (2008). Diatoms-from cell wall biogenesis to nanotechnology. Annu Rev Genet, 42(1): 83–107
Kuo-Huang L L, Ku M S B, Franceschi V R (2007). Correlations between calcium oxalate crystals and photosynthetic activites in palisade cells of shade-adapted Peperomia glabella. Bot Stud (Taipei, Taiwan), 48(2): 155–164
Kuo-Huang L L, Zindler-Frank E (1998). Structure of crystal cells and influences of leaf development on crystal cell development and vice versa in Phaseolus vulgaris (Leguminosae). Bot Acta, 111: 337–345
Lazzaro M D, Thomson W W (1989). Ultrastructure of organic acid secreting trichomes of chickpea (Cicer arietinum). Can J Bot, 67(9): 2669–2677
Leeuwenhoek A (1675). Microscopical observations. Philos T Roy Soc, 10: 380–385
Lersten N, Horner H (2008a). Crystal macropatterns in leaves of Fagaceae and Nothofagaceae: a comparative study. Plant Syst Evol, 271(3–4): 239–253
Lersten N, Horner H (2008b). Subepidermal idioblasts and crystal macropattern in leaves of Ticodendron (Ticodendraceae). Plant Syst Evol, 276(3–4): 255–260
Lersten N, Horner H (2009). Crystal diversity and macropatterns in leaves of Oleaceae. Plant Syst Evol, 282(1–2): 87–102
Lersten N R, Horner H T (2000). Types of calcium oxalate crystals and macro patterns in leaves of Prunus (Rosaceae: Prunoideae). Plant Syst Evol, 224: 83–96
Lersten N R, Horner H T (2011). Unique calcium oxalate “duplex” and “concretion” idioblasts in leaves of tribe Naucleeae (Rubiaceae). Am J Bot, 98(1): 1–11
Li X X, Franceschi V R (1990). Distribution of peroxisomes and glycolate metabolism in relation to calcium oxalate formation in Lemna minor L. Eur J Cell Biol, 51(1): 9–16
Li X X, Zhang D Z, Lynch-Holm V J, Okita T W, Franceschi V R (2003). Isolation of a crystal matrix protein associated with calcium oxalate precipitation in vacuoles of specialized cells. Plant Physiol, 133(2): 549–559
Libert B (1987). Breeding a low-oxalate rhubarb (Rheum sp. L.). J Hortic Sci Biotechnol, 62(4): 523–529
Libert B, Franceschi V R (1987). Oxalate in crop plants. J Agric Food Chem, 35(6): 926–938
Loewus F (1999). Biosynthesis and metabolism of ascorbic acid in plants and of analogs of ascorbic acid in fungi. Phytochemistry, 52(2): 193–210
Loewus F A, Wagner G, Yang J C (1975). Biosynthesis and metabolism of ascorbic acid in plants. Ann N YAcad Sci, 258(1 Second Confer): 7–23
Ma J F, Hiradate S, Nomoto K, Iwashita T, Matsumoto H (1997a). Internal detoxification mechanism of Al in hydrangea. Plant Physiol, 113(4): 1033–1039
Ma J F, Ryan P R, Delhaize E (2001). Aluminium tolerance in plants and the complexing role of organic acids. Trends Plant Sci, 6(6): 273–278
Ma J F, Zheng S J, Matsumoto H, Hiradate S (1997b). Detoxifying aluminium with buckwheat. Nature, 390(6660): 569–570
Massey L K, Palmer R G, Horner H T (2001). Oxalate content of soybean seeds (Glycine max: Leguminosae), soyfoods, and other edible legumes. J Agric Food Chem, 49(9): 4262–4266
Mazen A M A (2004). Calcium oxalate deposits in leaves of Corchorus olitotius as related to accumulation of toxic metals. Russ J Plant Physiol, 51(2): 281–285
Mazen A M A, Zhang D Z, Franceschi V R (2004). Calcium oxalate formation in Lemna minor: physiological and ultrastructural aspects of high capacity calcium sequestration. New Phytol, 161(2): 435–448
McConn M M, Nakata PA (2002). Calcium oxalate crystal morphology mutants from Medicago truncatula. Planta, 215(3): 380–386
McConn M M, Nakata PA (2004). Oxalate reduces calcium availability in the pads of the prickly pear cactus through formation of calcium oxalate crystals. J Agric Food Chem, 52(5): 1371–1374
McNair J B (1932). The interrelation between substances in plants: essential oils and resins, cyanogen and oxalate. Am J Bot, 19(3): 255–271
Melino V J, Soole K L, Ford C M (2009). Ascorbate metabolism and the developmental demand for tartaric and oxalic acids in ripening grape berries. BMC Plant Biol, 9(1): 145
Molano-Flores B (2001). Herbivory and calcium concentrations affect calcium oxalate crystal formation in leaves of Sida (Malvaceae). Ann Bot (Lond), 88(3): 387–391
Monje P V, Baran E J (2002). Characterization of calcium oxalates generated as biominerals in cacti. Plant Physiol, 128(2): 707–713
Moreau A G, Savage G P (2009). Oxalate content of purslane leaves and the effect of combining them with yoghurt or coconut products. J Food Compost Anal, 22(4): 303–306
Morris J, Nakata P A, McConn M, Brock A, Hirschi K D (2007). Increased calcium bioavailability in mice fed genetically engineered plants lacking calcium oxalate. Plant Mol Biol, 64(5): 613–618
Morrow A C, Dute R R (2002). Crystals associated with the intertracheid pit membrane of the woody fern Botrychium multifidum. Am Fern J, 92(1): 10–19
Nakata P A (2003). Advances in our understanding of calcium oxalate crystal formation and function in plants. Plant Sci, 164(6): 901–909
Nakata PA (2012). Influence of calcium oxalate crystal accumulation on the calcium content of seeds from Medicago truncatula. Plant Sci, 185–186(0): 246–249
Nakata P A, Kostman T A, Franceschi V R (2003). Calreticulin is enriched in the crystal idioblasts of Pistia stratiotes. Plant Physiol Biochem, 41(5): 425–430
Nakata P A, McConn M (2002). Sequential subtractive approach facilitates identification of differentially expressed genes. Plant Physiol Biochem, 40(4): 307–312
Nakata P A, McConn M M (2000). Isolation of Medicago truncatula mutants defective in calcium oxalate crystal formation. Plant Physiol, 124(3): 1097–1104
Nakata PA, McConn MM (2003a). Calcium oxalate crystal formation is not essential for growth of Medicago truncatula. Plant Physiol Biochem, 41(4): 325–329
Nakata P A, McConn M M (2003b). Influence of the calcium oxalate defective 4 (cod4) mutation on the growth, oxalate content, and calcium content of Medicago truncatula. Plant Sci, 164(4): 617–621
Nakata P A, McConn M M (2006). A genetic mutation that reduces calcium oxalate content increases calcium availability in Medicago truncatula. Funct Plant Biol, 33(7): 703–706
Nakata PA, McConn M M (2007a). Calcium oxalate content affects the nutritional availability of calcium from Medicago truncatula leaves. Plant Sci, 172(5): 958–961
Nakata PA, McConn M M (2007b). Genetic evidence for differences in the pathways of druse and prismatic calcium oxalate crystal formation in Medicago truncatula. Funct Plant Biol, 34(4): 332–338
Nakata P A, McConn M M (2007c). Isolated Medicago truncatula mutants with increased calcium oxalate crystal accumulation have decreased ascorbic acid levels. Plant Physiol Biochem, 45(3–4): 216–220
Nordin B E C, Hodgkinson A, Peacock M, Robertson W G (1979). Urinary tract calculi. In: Nephrology (Hamburger J, Crosnier J, Grunfeld J P, Eds). Wiley: New York and Paris, 1091
Nuss R F, Loewus F A (1978). Further studies on oxalic acid biosynthesis in oxalate-accumulating plants. Plant Physiol, 61(4): 590–592
Olszta M J, Cheng X, Jee S S, Kumar R, Kim Y Y, Kaufman M J, Douglas E P, Gower L B (2007). Bone structure and formation: A new perspective. Mater Sci Eng Rep, 58(3–5): 77–116
Oscarsson K V, Savage G P (2007). Composition and availability of soluble and insoluble oxalates in raw and cooked taro (Colocasia esculenta var. Schott) leaves. Food Chem, 101(2): 559–562
Park S H, Doege S J, Nakata P A, Korth K L (2009). Medicago truncatula-derived calcium oxalate crystals have a negative impact on chewing insect performance via their physical properties. Entomol Exp Appl, 131(2): 208–215
Parsons H T, Fry S C (2012). Oxidation of dehydroascorbic acid and 2,3-diketogulonate under plant apoplastic conditions. Phytochemistry, 75(0): 41–49
Parsons H T, Yasmin T, Fry S C (2011). Alternative pathways of dehydroascorbic acid degradation in vitro and in plant cell cultures: novel insights into vitamin C catabolism. Biochem J, 440(3): 375–383
Pennisi S V, McConnell D B (2001). Inducible calcium sinks and preferential calcium allocation in leaf primordia of Dracaena sanderiana Hort. Sander ex M.T. Mast. (Dracaenaceae). HortScience, 36: 1187–1191
Pennisi S V, McConnell D B, Gower L B, Kane M E, Lucansky T (2001). Intracellular calcium oxalate crystal structure in Dracaena sanderiana. New Phytol, 150(1): 111–120
Proietti S, Moscatello S, Famiani F, Battistelli A (2009). Increase of ascorbic acid content and nutritional quality in spinach leaves during physiological acclimation to low temperature. Plant Physiol Biochem, 47(8): 717–723
Prychid C J, Jabaily R S, Rudall P J (2008). Cellular ultrastructure and crystal development in Amorphophallus (Araceae). Ann Bot (Lond), 101(7): 983–995
Prychid C J, Rudall P J (1999). Calcium oxalate crystals in monocotyledons: A review of their structure and systematics. Ann Bot (Lond), 84(6): 725–739
Rahman M M, Ishii Y, Niimi M, Kawamura O (2010). Effect of application form of nitrogen on oxalate accumulation and mineral uptake by napiergrass (Pennisetum purpureum). Grassland Sci, 56(3): 141–144
Rinallo C, Modi G (2002). Content of oxalate in Actinidia deliciosa plants grown in nutrient solutions with different nitrogen forms. Biol Plant, 45(1): 137–139
Ritter M M C, Savage G P (2007). Soluble and insoluble oxalate content of nuts. J Food Compost Anal, 20(3–4): 169–174
Ruiz N, Ward D, Saltz S (2002a). Calcium oxalate crystals in leaves of Pancratium sickenbergeri: constitutive or induced defense? Funct Ecol, 16(1): 99–105
Ruiz N, Ward D, Saltz S (2002b). Responses of Pancratium sickenbergeri to simulated bulb herbivory: combining defence and tolerance strategies. J Ecol, 90(3): 472–479
Ryall R L, Stapleton A M F (1995) Urinary macromolecules in calcium oxalate stone and crystal matrix: good, bad, or indifferent? In: Calcium oxalate in biological systems (Kahn S R, Ed.). CRC Press, Inc.: Boca Raton, 265–290
Ryan P R, Delhaize E, Jones D L (2001). Function and mechanism of organic anion exudation from plant roots. Annu Rev Plant Physiol Plant Mol Biol, 52(1): 527–560
Saito K, Ohmoto J, Kuriha N (1997). Incorporation of 18O into oxalic, Lthreonic and L-tartaric acids during cleavage of L-ascorbic and 5-keto-D-gluconic acids in plants. Phytochemistry, 44(5): 805–809
Saltz S, Ward D (2000). Responding to a three-pronged attack: desert lilies subject to herbivory by dorcas gazelles. Plant Ecol, 148(2): 127–138
Savage G P, Mårtensson L, Sedcole J R (2009). Composition of oxalates in baked taro (Colocasia esculenta var. Schott) leaves cooked alone or with additions of cows milk or coconut milk. J Food Compost Anal, 22(1): 83–86
Savage G P, Vanhanen L, Mason S M, Ross A B (2000). Effect of cooking on the soluble and insoluble oxalate content of some New Zealand foods. J Food Compost Anal, 13(3): 201–206
Siener R, Hönow R, Seidler A, Voss S, Hesse A (2006a). Oxalate contents of species of the Polygonaceae, Amaranthaceae and Chenopodiaceae families. Food Chem, 98(2): 220–224
Siener R, Hönow R, Voss S, Seidler A, Hesse A (2006b). Oxalate content of cereals and cereal products. J Agric Food Chem, 54(8): 3008–3011
Smith K T, Shortle W C, Connolly J H, Minocha R, Jellison J (2009). Calcium fertilization increases the concentration of calcium in sapwood and calcium oxalate in foliage of red spruce. Environ Exp Bot, 67(1): 277–283
Sugiyama N, Okutani I (1996). Relationship between nitrate reduction and oxalate synthesis in spinach leaves. J Plant Physiol, 149(1–2): 14–18
Taylor G J (1991). Current views of the aluminum stress response; the physiological basis of tolerance. Curr Top Plant Biochem Physiol, 10: 57–93
Thongboonkerd V, Semangoen T, Chutipongtanate S (2006). Factors determining types and morphologies of calcium oxalate crystals: molar concentrations, buffering, pH, stirring and temperature. Clin Chim Acta, 367(1–2): 120–131
Thurston E L (1976). Morphology, fine structure and ontogeny of the stinging emergence of Tragia ramosa and T. saxicola (Euphorbiaceae). Am J Bot, 63(6): 710–718
Tillman-Sutela E, Kauppi A (1999). Calcium oxalate crystals in the mature seeds of Norway spruce, Picea abies (L.) Karst. Trees (Berl), 13(3): 131–137
Volk G M, Lynch-Holm V J, Kostman T A, Goss L J, Franceschi V R (2002). The role of druse and raphide calcium oxalate crystals in tissue calcium regulation in Pistia stratiotes leaves. Plant Biol, 4(1): 34–45
Wagner G, Loewus F (1973). The biosynthesis of (+)-tartaric acid in Pelargonium crispum. Plant Physiol, 52(6): 651–654
Ward D, Spiegel M, Saltz S (1997). Gazelle herbivory and interpopulation differences in calcium oxalate content of leaves of a desert lilly. J Chem Ecol, 23(2): 333–346
Weaver C M, Martin B R, Ebner J S, Krueger C A (1987). Oxalic acid decreases calcium absorption in rats. J Nutr, 117(11): 1903–1906
Webb M A (1999). Cell-mediated crystallization of calcium oxalate in plants. Plant Cell, 11(4): 751–761
Webb M A, Arnott H J (1981). An ultrastructural study of druse crystals in okra cotyledons. Scan Electron Microsc, 3: 285–292
Webb M A, Arnott H J (1983). Inside plant crystals: a study of the noncrystalline core in druses of Vitis vinifera endosperm. Scan Electron Microsc, IV: 1759–1770
Webb M A, Cavaletto J M, Carpita N C, Lopez L E, Arnott H J (1995). The intravacuolar organic matrix associated with calcium oxalate crystals in leaves of Vitis. Plant J, 7(4): 633–648
Weiner S, Addadi L (1991). Acidic macromolecules of mineralized tissues: the controllers of crystal formation. Trends Biochem Sci, 16(7): 252–256
Xu HW, Ji X M, He Z H, ShiW P, Zhu G H, Niu J K, Li B S, Peng X X (2006). Oxalate accumulation and regulation is independent of glycolate oxidase in rice leaves. J Exp Bot, 57(9): 1899–1908
Yang J C, Loewus F A (1975). Metabolic conversion of L-ascorbic acid in oxalate-accumulating plants. Plant Physiol, 56(2): 283–285
Yang Y Y, Jung J Y, Song W Y, Suh H S, Lee Y (2000). Identification of rice varieties with high tolerance or sensitivity to lead and characterization of the mechanism of tolerance. Plant Physiol, 124(3): 1019–1026
Yu L, Jiang J, Zhang C, Jiang L, Ye N, Lu Y, Yang G, Liu E, Peng C, He Z, Peng X (2010). Glyoxylate rather than ascorbate is an efficient precursor for oxalate biosynthesis in rice. J Exp Bot, 61(6): 1625–1634
Zindler-Frank E (1975). On the formation of the pattern of crystal idioblasts in Canavalia ensiformis D.C.: VII. Calcium and oxalate content of the leaves in dependence of calcium nutrition. Z Pflanzenphysiol, 77: 80–85
Zindler-Frank E (1976). Oxalate biosynthesis in relation to photosynthetic pathways and plant productivity: a survey. Z Pflanzenphysiol, 80: 1–13
Zindler-Frank E (1987) Calcium oxalate in legumes. In: Advances in Legume Systematics (Stirton E, Ed.)Royal Botanic Gardens: Kew, UK, 279–316
Zindler-Frank E (1991). Calcium oxalate crystal formation and growth in two legume species as altered by strontium. Bot Acta, 104: 229–232
Zindler-Frank E, Honow R, Hesse A (2001). Calcium and oxalate content of the leaves of Phaseolus vulgaris at different calcium supply in relation to calcium oxalate crystal formation. J Plant Physiol, 158(2): 139–144
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Nakata, P.A. Plant calcium oxalate crystal formation, function, and its impact on human health. Front. Biol. 7, 254–266 (2012). https://doi.org/10.1007/s11515-012-1224-0
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DOI: https://doi.org/10.1007/s11515-012-1224-0