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Gold Nanoparticle Uptake by Tumour Cells of B16 Mouse Melanoma

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Abstract

Because of their photo-optical distinctiveness and biocompatibility, gold nanoparticles have proven to be powerful tools in various nanomedical applications. In this article, we discuss the advantage of gold nanoparticles in image diagnostic application of melanoma. It has demonstrated the potential role of gold nanoparticles in the study of tumour tissue architecture and the utility of gold nanoparticles in the hystopathological exam of B16 melanoma with the benefit of fluorescence emission of gold nanoparticles in UV spectrum. The optical properties of colloidal gold nanoparticles allow spectroscopic detection and identification of melanoma cells. The method proposed is easy, inexpensive and reliable for hystopathological analysis of melanoma. The fluorescence images in the cryosections of tissues depicted a strong luminescence property of gold nanoparticles uptaken in melanoma, results that confirm the role of the gold nanoparticles in biological labelling and imaging applications. To emphasize the AuNPs influence over the biological tissues, a study of the chemical bonds configuration was performed using Raman spectrometry.

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Acknowledgments

The authors wish to acknowledge the financial support from the European Social Fund through POSDRU/89/1.5/S/63700, 2010–2013, ‘Human Resource Development by Postdoctoral Research on Micro and Nanotechnologies’, and the Project No. 582/2009—PN II ‘Ideas’ Program, funded under PN II Program of the Romanian Ministry of Education, Research, Youth and Sport–National Authority for Scientific Research. The authors appreciate the help of PhD student Ana Maria Enciu from the National Institute for Research and Development in the Field of Pathology and Biomedical Sciences Victor Babeş, Bucharest, Romania and George Simion from the Department of Pathology of Emergency University Hospital, Bucharest, Romania. The work was carried out in collaboration with the Department of Molecular Biology from the National Institute Victor Babeş, Bucharest, Romania.

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Authors and Affiliations

  1. L10 Micro and Nano Fluidics Laboratory, National Institute for Research and Development in Microtechnologies, 126A Erou Iancu Nicolae Street, Bucharest, Romania

    Marioara Avram, Cătălin Mihai Bălan, Vasilica Schiopu, Cătălin Mărculescu & Andrei Avram

  2. Department of Plastic Surgery and Reconstructive Microsurgery, Emergency University Hospital, 169 Splaiul Independentei, Bucharest, Romania

    Ina Petrescu

  3. Politehnica University of Bucharest, 313 Splaiul Independentei, Bucharest, Romania

    Andrei Avram

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  1. Marioara Avram
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  2. Cătălin Mihai Bălan
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  3. Ina Petrescu
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  4. Vasilica Schiopu
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  5. Cătălin Mărculescu
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Correspondence to Cătălin Mihai Bălan.

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Avram, M., Bălan, C.M., Petrescu, I. et al. Gold Nanoparticle Uptake by Tumour Cells of B16 Mouse Melanoma. Plasmonics 7, 717–724 (2012). https://doi.org/10.1007/s11468-012-9363-3

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  • DOI: https://doi.org/10.1007/s11468-012-9363-3

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