Abstract
Three allelic short root mutants were identified by screening mutants with defective root elongation of the rice japonica cultivar Nipponbare mutant library generated via 60Co γ-ray irradiation mutagenesis. These mutants, designated srt7-1 (short root 7-1), srt7-2 and srt7-3, respectively, had an extremely short seminal root, adventitious roots and lateral roots. Histological observation revealed the cell length of srt7 mutant roots was significantly shorter than that of wild-type roots. Genetic analysis indicated the short root phenotype was controlled by a single recessive nuclear gene. The SRT7 gene was mapped to a 20-kb interval between the markers STS6 and STS7 on chromosome 4 by a map-based cloning method. Sequencing of the six predicted genes in this region found that all of the three allelic mutants contained a 1-bp or 2-bp deletion in the same gene encoding a putative membrane-bound endo-1,4-β-glucanase. The SRT7 gene was expressed ubiquitously, with higher levels of transcript accumulation in roots at different developmental stages. However, no difference was found in the SRT7 transcription level between the mutant and wild type. Collectively, these results indicate the endo-1,4-β-glucanase encoding gene (LOC_Os04g41970) is likely the candidate for SRT7 that functions posttranscriptionally in rice root elongation.
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References
Lynch J. Root architecture and plant productivity. Plant Physiol, 1995, 109: 7–13
Beemster G T, Fiorani F, Inze D. Cell cycle: The key to plant growth control? Trends Plant Sci, 2003, 8: 154–158
Ichii M, Ishikawa M. Genetic analysis of newly induced short-root mutants in rice (Oryza sativa L.). Breed Sci, 1997, 47: 121–125
Inukai Y, Miwa M, Nagato Y, et al. RRL1, RRL2 and CRL2 loci regulating root elongation in rice. Breed Sci, 2001, 51: 231–239
Yao S G, Taketa S, Ichii M. A novel short-root gene that affects specifically early root development in rice (Oryza sativa L.). Plant Sci, 2002, 163: 207–215
Yao S G, Taketa S, Ichii M. Isolation and characterization of an abscisic acid insensitive mutation that affects specifically primary root elongation in rice (Oryza sativa L.). Plant Sci, 2003, 164: 971–978
Yao S G, Kodama R, Wang H, et al. Analysis of the rice SHORTROOT5 gene revealed functional diversification of plant neutral/alkaline invertase family. Plant Sci, 2009, 176: 627–634
Hochholdinger F, Park W J, Sauer M, et al. From weeds to crops: Genetic analysis of root development in cereals. Trends Plant Sci, 2004, 9: 42–48
Miwa M, Inukai Y, Itoh M, et al. Genetic mapping of a short root mutant in rice. Breeding Res, 2000, 2(Suppl 1): 56
Ning Y Q, Ding W N, Zhu S H, et al. Genetic analysis and gene mapping of a short root mutant ksr1 in rice. Chin J Rice Sci, 2010, 24: 652–654
Jiang H W, Wang S M, Dang L, et al. A novel short-root gene encodes a glucosamine-6-phosphate acetyltransferase required for maintaining normal root cell shape in rice. Plant Physiol, 2005, 138: 232–242
Li J, Zhu S H, Song X W, et al. A rice glutamater receptor-like gene is critical for the division and survival of individual cells in the root apical meristem. Plant Cell, 2006, 18: 340–349
Jia L, Zhang B, Mao C, et al. OsCYT-INV1 for alkaline/neutral invertase is involved in root cell development and reproductivity in rice (Oryza sativa L.). Planta, 2008, 228: 51–59
Jia L, Wu Z, Hao X, et al. Identification of a novel mitochondrial protein, short postembryonic roots 1 (SPR1), involved in root development and iron homeostasis in Oryza sativa. New Phytol, 2011, 189: 843–855
Molhoj M, Ulvskov P, Dal D F. Characterization of a functional soluble form of a Brassica napus membrane-anchored endo-1,4-betaglucanase heterologously expressed in Pichia pastoris. Plant Physiol, 2001, 127: 674–684
Nicol F, His I, Jauneau A, et al. A plasma membrane-bound putative endo-1,4-beta-D-glucanase is required for normal wall assembly and cell elongation in Arabidopsis. EMBO J, 1998, 17: 5563–5576
Zuo J, Niu Q W, Nishizawa N, et al. KORRIGAN, an Arabidopsis endo-1,4-glucanase, localizes to the cell plate by polarized targeting and is essential for cytokinesis. Plant Cell, 2000, 12: 1137–1152
Shani Z, Dekel M, Tsabary G, et al. Cloning and characterization of elongation specific endo-1,4-betaglucanase (cel1) from Arabidopsis thaliana. Plant Mol Biol, 1997, 34: 837–842
Tsabary G, Shani Z, Roiz L, et al. Abnormal ‘wrinkled’ cell walls and retarded development of transgenic Arabidopsis thaliana plants expressing endo-1,4-beta-glucanase (cell) antisense. Plant Mol Biol, 2003, 51: 213–224
Libertini E, Li Y, McQueen-Mason S J. Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family. J Mol Evol, 2004, 58: 506–515
Zhou H L, He S J, Cao Y R, et al. OsGLU1, a putative membranebound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation. Plant Mol Biol, 2006, 60: 137–151
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Liu, H., Zheng, H., Wang, H. et al. Fine-mapping of SRT7 for short roots and identification of its candidate in rice. Chin. Sci. Bull. 56, 3296–3300 (2011). https://doi.org/10.1007/s11434-011-4776-5
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DOI: https://doi.org/10.1007/s11434-011-4776-5