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Functional analysis of the sbcD (dr1921) gene of the extremely radioresistant bacterium Deinococcus radiodurans

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  • Microbiology
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Chinese Science Bulletin

Abstract

In eukaryotes, the Mre11-Rad50-Nbs1 (MRN) complex, which resides at the crossroads of DNA repair and checkpoint signaling, rapidly forms prominent foci at damage sites following double-strand break (DSB) induction. This complex carries out the initial processing of the DSB ends. Mutations in the genes that encode components of this complex result in DNA-damage hypersensitivity, genomic instability, telomere shortening, and aberrant meiosis. Therefore, the MR proteins are highly conserved during evolution. The bacterial orthologs of Mre11 and Rad50 are the SbcD and SbcC proteins, respectively. Deinococcus radiodurans, an extremely radioresistant bacterium, is able to mend hundreds of radiation-induced DSBs. The SbcD and SbcC proteins were identified as the products of the Dr1921 and Dr1922 genes. Disruption of the sbcD gene, by direct reverse-orientation insertional mutagenesis technology, remarkably increases the cells’ sensitivity to various types of DNA damaging agents, such as ionizing radiation, ultraviolet irradiation, hydrogen peroxide, and mitomycin C. We also provide evidence that the drSbcD protein plays an important role in both growth and DNA repair in this organism, especially in repair of DSBs generated after cellular exposure to 6000 Gy of IR. These results demonstrate that the drSbcD protein plays an important role in DSBs repair in D. radiodurans.

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References

  1. Anderson A M, Nordon H C, Cain R F, et al. Studies on a radioresistant micrococcus. 1. Isolation, morphology, cultural characteristics, and resistance to γ radiation. Food Technol, 1956, 10: 575–578

    Google Scholar 

  2. Cox M M, Battista J R. Deinococcua radiodurans-the consummate suvivor. Nat Rev Microbiol, 2005, 3: 882–892

    Article  Google Scholar 

  3. Allen C, Halbrook J, Nickoloff J A. Interactive competition between homologous recombination and non-homologous end joining. Mol Cancer Res, 2003, 1: 913–920

    Google Scholar 

  4. Haber J E. Parters and pathways repairing a double-strand break. Trends Genet, 2000, 16: 259–264

    Article  Google Scholar 

  5. Wilson T E, Topper L M, Palmbos P L. Non-homologous end-joining: Bacteria join the chromosome breakdance. Trends Biochem Sci, 2003, 28: 62–66

    Article  Google Scholar 

  6. Rocher E P C, Corner E, Michel B. Comparative and evolutionary analysis of the bacterial homologous recombination. PLoS Genet, 2005, 1: 0247–0259

    Google Scholar 

  7. Hopfnor K, Putnam C D, Tainer J A. DNA double-strand break repair from head to tail. Curr Opin Struct Biol, 2002, 12: 115–122

    Article  Google Scholar 

  8. Shiloh Y. ATM and related protein kinases: Safeguarding genome integrity. Nat Rev Cancer, 2003, 3: 156–167

    Article  Google Scholar 

  9. Kaye J A, Melo J A, Cheung S K, et al. DNA breaks promote genomic instability by impeding proper chromosome segregation. Curr Biol, 2004, 14: 2096–2106

    Article  Google Scholar 

  10. Lobachev K, Vitriol E, Stemple J, et al. Chromosome fragmentation after induction of a double-strand break is an active process prevent by the RMX repair complex. Curr Biol, 2004, 14: 2107–2112

    Article  Google Scholar 

  11. D’Amours D, Jackson S P. The Mre11 complex: At the crossroads of DNA repair and checkpoint signalling. Nat Rev Mol Cell Biol, 2002, 3: 317–327

    Article  Google Scholar 

  12. Assenmacher N, Hopfner K. Mre11/Rad50/Nbs1: complex activities. Chromosoma, 2004, 113: 157–166

    Article  Google Scholar 

  13. Eisen J A, Hanawalt P C. A phylogenomic study of DNA repair genes, proteins, and processes. DNA Repair, 1999, 435: 171–213

    Google Scholar 

  14. Kuzminov A. Recombinational repair of DNA damage in Escherichia coli and Bacteriophage λ. Microbiol Mol Biol R, 1999, 63: 751–813

    Google Scholar 

  15. Bidnenko V, Seigneur M, Penel-Colin M, et al. sbcB sbcC null mutations allow RecF-mediated repair of arrested replication forks in rep recBC mutants. Mol Microbiol, 1999, 33: 846–857

    Article  Google Scholar 

  16. Yamaguchi H, Hanada K, Asami Y, et al. Control of genetic stability in Escherichia coli: The SbcB 3′–5′ exonuclease suppresses illegitimate recombination promoted by the RecE 3′–5′ exonuclease. Genes Cells, 2000, 5: 101–109

    Article  Google Scholar 

  17. Shiraishi K, Hanada K, Iwakura Y, et al. Roles of RecJ, RecO, and RecR in RecET-mediated illegitimate recombination in Escherichia coli. J Bacteriol, 2002, 184: 4715–4721

    Article  Google Scholar 

  18. Kikuchi M, Narumi I, Kitayama S, et al. Genomic organization of the radioresistant bacterium Deinococcus radiodurans: Physical map and evidence for multiple replicons. FEMS Microbiol Lett, 1999, 174: 151–157

    Article  Google Scholar 

  19. Connelly J C, Leau E, Leach D R F. DNA cleavage and degradation by the SbcCD protein complex from Escherichia coli. Nucleic Acid Res, 1999, 27: 1039–1046

    Article  Google Scholar 

  20. Connelly J C, Leau E, Leach D R F. Nucleolytic processing of a protein-bound DNA end by the E. coli SbcCD (MR) complex. DNA Repair, 2003, 2: 795–807

    Article  Google Scholar 

  21. Connelly J C, Kirkham L A, Leach D R F. The SbcCD nuclease of Escherichia coli is a structural maintenance of chromosomes (SMC) family protein that cleaves hairpin DNA. Proc Natl Acad Sci USA, 1998, 95: 7969–7974

    Article  Google Scholar 

  22. Cromie G A, Leach D R F. Recombinational repair of chromosomal DNA double-strand breaks generated by a restriction endonuclease. Mol Microbiol, 2001, 41: 873–883

    Article  Google Scholar 

  23. White O, Eisen J A, Heidelberg J F, et al. Genome sequence of the radioresistant bacterium Deinococcus radiodurans. Science, 1999, 286: 1571–1577

    Article  Google Scholar 

  24. Makarova K S, Aravind L, Wolf Y I, et al. Genome of the extremely radioation-resistant bacterium Deinococcus radiodurans viewed from the perspective of comparative genomics. Microbiol Mol Biol R, 2001, 65: 44–79

    Article  Google Scholar 

  25. Connelly J C, Leach D R F. Tethering on the brink: The evolutionarily conserved Mre11-Rad50 complex. Trends Biochem Sci, 2002, 27: 410–418

    Article  Google Scholar 

  26. Funayama T, Narumi I, Kikuchi M, et al. Identification and disruption analysis of the recN gene in the extremely radioresistant bacterium Deinococcus radiodurans. DNA Repair, 1999, 435: 151–161

    Google Scholar 

  27. Harris D R, Tanaka M, Saveliev S V, et al. Preserving genome integrity: The DdrA protein of Deinococcus radiodurans R1. PLoS Biol, 2004, 2: 1629–1639

    Article  Google Scholar 

  28. Jager M, Trujillo K M, Sung P, et al. Differential arrangements of conserved building blocks among homologs of the Rad50/Mre11 DNA repair protein complex. J Mol Biol, 2004, 339: 937–979

    Article  Google Scholar 

Download references

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Correspondence to Hua YueJin.

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Supported by the National Basic Research Program of China (Grant No. 2004CB19604), National Science Fund for Distinguished Young Scholars (Grant No. 30425038), and Key Project from the National Natural Science Foundation of China (Grant No. 30330020)

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Hu, Y., Ma, C., Tian, B. et al. Functional analysis of the sbcD (dr1921) gene of the extremely radioresistant bacterium Deinococcus radiodurans . CHINESE SCI BULL 52, 2506–2513 (2007). https://doi.org/10.1007/s11434-007-0382-y

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  • DOI: https://doi.org/10.1007/s11434-007-0382-y

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