The impact of space environment on gene expression in Arabidopsis thaliana seedlings

  • HuaSheng Li
  • JinYing Lu
  • Hui Zhao
  • Qiao Sun
  • FuTong Yu
  • Yi Pan
  • Yu Chen
  • Liang Su
  • Min Liu
Article

DOI: 10.1007/s11431-016-0232-7

Cite this article as:
Li, H., Lu, J., Zhao, H. et al. Sci. China Technol. Sci. (2017). doi:10.1007/s11431-016-0232-7
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Abstract

One of the important questions in space biology is the mechanisms underlying plant responses to an outer space environment, i.e., how gene expression is altered in space. In this study, the transcriptome of Arabidopsis thaliana seedlings was analyzed as a part of Germany SIMBOX (science in microgravity box) spaceflight experiment on Shenzhou 8 spacecraft. This experiment involved the following treatments: spaceflight with microgravity (F μg), spaceflight with 1g centrifugal force (F 1g), and ground 1g control (G 1g). Gene chips were used to screen gene expression differences in Arabidopsis thaliana seedlings among these treatments. Microarray analysis revealed that 621 genes were differentially expressed in samples F μg vs. G 1g, 249 genes in samples F 1g vs. G 1g, and 368 genes in samples F μg vs. F 1g. Gene ontology analysis indicated that the genes were involved in metabolism of stress response, gravitropic response, and DNA damage and repair, suggesting that plants adjust these metabolic pathways to space environmental stress, microgravity, and radiation.

Keywords

Arabidopsis thaliana space flight microgravity microarray gene expression profile 

Supplementary material

11431_2016_232_MOESM1_ESM.doc (99 kb)
Differentially expressed genes (fold change >5) in samples of three treatments. Note: “a” represents 18 common genes in three treatments which expression were significant changed. “-” represents cannot get the reliable value
11431_2016_232_MOESM2_ESM.doc (216 kb)
Agarose gel analysis of extracted RNA from ground control (G 1g) on-board 1g centrifuge (F 1g), spaceflight (F μg) samples, front and rear CC (Culture Chamber)

Copyright information

© Science China Press and Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • HuaSheng Li
    • 1
  • JinYing Lu
    • 1
  • Hui Zhao
    • 1
  • Qiao Sun
    • 1
  • FuTong Yu
    • 2
  • Yi Pan
    • 3
  • Yu Chen
    • 1
  • Liang Su
    • 1
  • Min Liu
    • 1
    • 3
  1. 1.Space Molecular Biological LaboratoryChina Academy of Space TechnologyBeijingChina
  2. 2.Department of Plant NutritionChina Agricultural UniversityBeijingChina
  3. 3.Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina

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