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Protective effects of aloperine on neonatal rat primary cultured hippocampal neurons injured by oxygen–glucose deprivation and reperfusion

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Abstract

Aloperine (ALO), one of the alkaloids isolated from Sophora alopecuroides L., is traditionally used for various diseases including neuronal disorders. This study investigated the protective effects of ALO on neonatal rat primary-cultured hippocampal neurons injured by oxygen–glucose deprivation and reperfusion (OGD/RP). Treatment with ALO (25, 50, and 100 mg/l) attenuated neuronal damage (p < 0.01), with evidence of increased cell viability (p < 0.01) and decreased cell morphologic impairment. Furthermore, ALO increased mitochondrial membrane potential (p < 0.01), but inhibited intracellular-free calcium [Ca2+] i (p  < 0.01) elevation in a dose-dependent manner at OGD/RP. ALO also reduced the intracellular reactive oxygen species and malondialdehyde production and enhanced the antioxidant enzymatic activities of catalase, superoxide dismutase, glutathione peroxidase and the total antioxidant capacity. The results suggested that ALO has significant neuroprotective effects that can be attributed to anti-oxidative stress.

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Acknowledgments

This study was supported by Ningxia Hui Autonomous Region, colleges and universities of science and technology research projects (NGY2013073). We are indebted to the staff in the animal center and the Science and Technology Center who provided assistance in the study.

Conflict of interest

The authors declare no conflict of interest.

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    Authors and Affiliations

    1. Department of Pharmacology, School of Pharmacy, Ningxia Medical University, Yinchuan, 750004, China

      Ning-Tian Ma, Ru Zhou, Ren-Yuan Chang, Yin-Ju Hao, Lin Ma, Shao-Ju Jin, Juan Du, Jie Zheng & Jian-Qiang Yu

    2. Key Lab of Fertility Preservation and Maintenance, Ministry of Education, Ningxia Medical University, Yinchuan, 750004, China

      Cheng-Jun Zhao

    3. School of Traditional Chinese Medicine, Ningxia Medical University, Yinchuan, 750004, China

      Yang Niu

    4. Ningxia Key Lab for Research on Craniocerebral Diseases of Ningxia Hui Autonomous Region, Yinchuan, 750004, China

      Tao Sun

    5. Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi, Chiba, 274-8510, Japan

      Wei Li & Kazuo Koike

    6. School of Nursing, Ningxia Medical University, Yinchuan, 750004, China

      Yu-Xiang Li

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    1. Ning-Tian Ma
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    Correspondence to Wei Li or Jian-Qiang Yu.

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    N.-T. Ma, R. Zhou, R.-Y. Chang contributed equally to this work.

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    Ma, NT., Zhou, R., Chang, RY. et al. Protective effects of aloperine on neonatal rat primary cultured hippocampal neurons injured by oxygen–glucose deprivation and reperfusion. J Nat Med 69, 575–583 (2015). https://doi.org/10.1007/s11418-015-0928-2

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    • DOI: https://doi.org/10.1007/s11418-015-0928-2

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