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Pharmacokinetics study of asperosaponin VI and its metabolites cauloside A, HN saponin F and hederagenin

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Abstract

This experiment's with aim was to study the pharmacokinetics of asperosaponin VI and its three metabolites (cauloside A, HN saponin F and hederagenin) via a sensitive high performance liquid chromatography connected with electrospray ionization triple quadrupole mass spectrum (HPLC–ESI–MS/MS). Chromatographic separation was achieved on a reverse phase C18 column with a gradient mobile phase of CH3CN-water with 0.1 % HCOOH at a flow rate of 0.3 mL/min. Sample analysis was simultaneously performed with a multiple reaction monitoring mode using target determination ions at m/z 927.5 → 603.4 for asperosaponin VI, m/z 811.1 → 603.4 for cauloside A, m/z 649.4 → 603.4 for HN saponin F, m/z 71.4 → 393.3 for hederagenin and m/z 307.0 → 161.1 for warfarin as the internal standard. The calibration curve was linear at the range of 0.25–500 ng/mL, and the lower limit of quantification was 0.25 ng/mL for each compound. While the precisely intra-assay and inter-assay variabilities were <9.5 and 7.8 %, respectively; accuracy was determined at the concentrations of 5, 25, 100 ng/mL for all the analytes with the relative standard deviation (%) no more than 15.0 %. Consequently, the validated method could be successfully and precisely applied to the pharmacokinetic study of asperosaponin VI and its metabolites. As a result, the pharmacokinetic parameters of cauloside A, HN saponin F and hederagenin such as T max were obtained at 9.33 ± 2.49, 7.33 ± 0.47 and 12.33 ± 2.36 h, respectively.

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Acknowledgments

This research was supported by Program for Important Drug Development of MOST, China (2012ZX09304007), 2013ZX09401-004-002, and 2011ZX09307-002-01).

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Authors and Affiliations

  1. Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, 88 Yuquan Road, Nankai District, Tianjin, 300193, China

    Er-Wei Liu, Jia-Long Wang & Li-Feng Han

  2. Tianjin University of Traditional Chinese Medicine, 88 Yuquan Road, Nankai District, Tianjin, 300193, China

    Yan-Xu Chang, Yan Huo & Lei Wang

  3. Tianjin State Key Laboratory of Modern Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin, 300193, China

    Tao Wang

  4. Tianjin Key Laboratory of Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, 88 Yuquan Road, Nankai District, Tianjin, 300193, China

    Xiu-Mei Gao

  5. Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Tianjin, China

    Xiu-Mei Gao

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  1. Er-Wei Liu
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  2. Jia-Long Wang
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  3. Li-Feng Han
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  4. Yan-Xu Chang
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  5. Tao Wang
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  7. Lei Wang
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Correspondence to Xiu-Mei Gao.

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Liu, EW., Wang, JL., Han, LF. et al. Pharmacokinetics study of asperosaponin VI and its metabolites cauloside A, HN saponin F and hederagenin. J Nat Med 68, 488–497 (2014). https://doi.org/10.1007/s11418-014-0821-4

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  • DOI: https://doi.org/10.1007/s11418-014-0821-4

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