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Effect of eurycomanone on cytochrome P450 isoforms CYP1A2, CYP2A6, CYP2C8, CYP2C9, CYP2C19, CYP2E1 and CYP3A4 in vitro

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Abstract

Eurycomanone, an active constituent isolated from Eurycoma longifolia Jack, was examined for modulatory effects on cytochrome P450 (CYP) isoforms CYP1A2, CYP2A6, CYP2C8, CYP2C9, CYP2C19, CYP2E1 and CYP3A4 using in vitro assays. The IC50 value was determined to assess the potencies of modulation for each CYP isoform. Our results indicated that eurycomanone did not potently inhibit any of the CYP isoforms investigated, with IC50 values greater than 250 μg/ml. Hence there appears to be little likelihood of drug–herb interaction between eurycomanone or herbal products with high content of this compound and CYP drug substrates via CYP inhibition.

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Acknowledgments

The authors thank the Malaysian Ministry of Science, Technology and Innovation (Grant: eScienceFund 02-02-09-SF0005) for kind funding and support given to this study.

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Authors and Affiliations

  1. Jeffrey Cheah School of Medicine and Health Sciences, Monash University Sunway Campus, Jalan Lagoon Selatan, 46150, Bandar Sunway, Selangor, Malaysia

    Chin Eng Ong

  2. School of Medical Sciences, International Medical University, 126 Jalan 19/155B, Bukit Jalil, 57000, Kuala Lumpur, Malaysia

    Yan Pan, Kai Hung Tiong & Joon Wah Mak

  3. Herbal Medicine Research Unit, Division of Biochemistry, Institute for Medical Research, Jalan Pahang, 50588, Kuala Lumpur, Malaysia

    Badrul Amini Abd-Rashid & Zakiah Ismail

  4. Pharmacogenetics Research Group, Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia

    Rusli Ismail

Authors
  1. Yan Pan
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  2. Kai Hung Tiong
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  3. Badrul Amini Abd-Rashid
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  4. Zakiah Ismail
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  7. Chin Eng Ong
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Correspondence to Chin Eng Ong.

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Pan, Y., Tiong, K.H., Abd-Rashid, B.A. et al. Effect of eurycomanone on cytochrome P450 isoforms CYP1A2, CYP2A6, CYP2C8, CYP2C9, CYP2C19, CYP2E1 and CYP3A4 in vitro. J Nat Med 68, 402–406 (2014). https://doi.org/10.1007/s11418-013-0794-8

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  • DOI: https://doi.org/10.1007/s11418-013-0794-8

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