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Polypharmacy (herbal and synthetic drug combination): a novel approach in the treatment of type-2 diabetes and its complications in rats

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Abstract

The aim of present study was to evaluate the effect of aqueous leaf extract of Annona squamosa with Glipizide in a high fat diet and streptozocin-induced type-2 diabetes. Nine groups (n = 6) of male Sprague-Dawley rats were used for the study, with. Basal blood glucose, urine volume, and body weights were measured and the rate were kept on a high fat diet. After 15 days, streptozocin in sub-diabetic dose (35 mg/kg) was administered to the animals to induce diabetes. With 1 week of consistent hyperglycemia, treatment was initiated. Aqueous extract of Annona squamosa was administered orally at 350 mg/kg body weight alone and in combination with reduced and reducing dose combinations of Glipizide. Blood glucose, body weight, urine volume were measured every 10th day. The elevated blood-glucose level in diabetic rats was controlled better with combination therapy compared with the synthetic drug alone or the herbal stand-alone drug. All the results were statistically significant (P < 0.001). A combination of Annona squamosa along with Glipizide may be helpful in dose reduction of Glipizide up to 50 %, reducing the risk of the onset of insulin therapy.

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Authors and Affiliations

  1. Siddhartha Institute of Pharmacy, Dehra Dun, 248001, Uttarakhand, India

    Rupinder Kaur, Muhammad Afzal, Imran Kazmi & Firoz Anwar

  2. Department of Pharmaceutics, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India

    Iqbal Ahamd

  3. Sebai Institute of Health Sciences, Jeddah, Kingdom of Saudi Arabia

    Zubair Ahmed

  4. Department of Pharmacognosy, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India

    Babar Ali & Sayeed Ahmad

  5. Pacific University, Udaipur, Rajasthan, India

    Rupinder Kaur

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  1. Rupinder Kaur
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  2. Muhammad Afzal
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  3. Imran Kazmi
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  4. Iqbal Ahamd
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  5. Zubair Ahmed
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  8. Firoz Anwar
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Correspondence to Muhammad Afzal or Firoz Anwar.

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Kaur, R., Afzal, M., Kazmi, I. et al. Polypharmacy (herbal and synthetic drug combination): a novel approach in the treatment of type-2 diabetes and its complications in rats. J Nat Med 67, 662–671 (2013). https://doi.org/10.1007/s11418-012-0720-5

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  • DOI: https://doi.org/10.1007/s11418-012-0720-5

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