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Studies on chemical fingerprints of Siraitia grosvenorii fruits (Luo Han Guo) by HPLC

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Abstract

A novel, efficient, and accurate fingerprinting method using high performance liquid chromatography–photodiode array detection has been developed and optimized for the investigation and demonstration of the variance in chemical properties among Siraitia grosvenorii fruits from different origins. The effects of growth stages, cultivated varieties, collection locations, and fruit portions of the herb on chromatographic fingerprints were examined. Eleven compounds were identified on chromatograms by comparing the retention time and UV spectrum of each peak separately with those of external references. The results revealed that chromatographic fingerprints, combining similarity or hierarchical clustering analysis along with reference compounds, could efficiently identify and distinguish S. grosvenorii fruits from different sources, which provided helpful clues for studying the plants’ secondary metabolites and benefitted quality control.

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Acknowledgment

This work was financial supported by a project of the Technology Foundation of Guilin (20070304-2). The project was also supported by West Light Doctoral Foundation of The Chinese Academy of Sciences. The authors would like to acknowledge Mr. Shuiyuan Jiang for his friendly help in collecting S. grosvenorii samples.

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Authors and Affiliations

  1. Guangxi Institute of Botany, Guangxi Zhuangzu Autonomous Region and Chinese Academy of Science, 541006, Guilin, China

    Fenglai Lu, Dianpeng Li, Chuanming Fu, Jinlei Liu, Yonglin Huang, Yueyuan Chen & Yongxin Wen

  2. Faculty of Pharmaceutical Sciences, Sojo University, 22-1 4-Chome, Ikeda, Kumamoto, 860-0082, Japan

    Toshihiro Nohara

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  1. Fenglai Lu
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  2. Dianpeng Li
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  3. Chuanming Fu
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  4. Jinlei Liu
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  5. Yonglin Huang
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  6. Yueyuan Chen
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  7. Yongxin Wen
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  8. Toshihiro Nohara
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Correspondence to Dianpeng Li.

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Lu, F., Li, D., Fu, C. et al. Studies on chemical fingerprints of Siraitia grosvenorii fruits (Luo Han Guo) by HPLC. J Nat Med 66, 70–76 (2012). https://doi.org/10.1007/s11418-011-0555-5

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  • DOI: https://doi.org/10.1007/s11418-011-0555-5

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