Journal of Natural Medicines

, Volume 65, Issue 1, pp 149–156

Anti-angiogenic effect of triterpenoidal saponins from Polygala senega

Authors

  • Masayoshi Arai
    • Graduate School of Pharmaceutical SciencesOsaka University
  • Asami Hayashi
    • Graduate School of Pharmaceutical SciencesOsaka University
  • Mari Sobou
    • Graduate School of Pharmaceutical SciencesOsaka University
  • Shunsuke Ishida
    • Graduate School of Pharmaceutical SciencesOsaka University
  • Takashi Kawachi
    • Graduate School of Pharmaceutical SciencesOsaka University
  • Naoyuki Kotoku
    • Graduate School of Pharmaceutical SciencesOsaka University
    • Graduate School of Pharmaceutical SciencesOsaka University
Original Paper

DOI: 10.1007/s11418-010-0477-7

Cite this article as:
Arai, M., Hayashi, A., Sobou, M. et al. J Nat Med (2011) 65: 149. doi:10.1007/s11418-010-0477-7

Abstract

Senegasaponins [senegin II (1), senegin III (2), senegin IV (3), senegasaponin a (4), and senegasaponin b (5)] from Polygala senega were re-discovered as selective anti-proliferative substances against human umbilical vein endothelial cells (HUVECs). Senegasaponins (15) showed anti-proliferative activity against HUVECs with IC50 values in the range 0.6–6.2 μM, and the selective index was 7–100-fold in comparison with those for several cancer cell lines, while the desacyl mixture of senegasaponins (6) and tenuifolin (7) lost anti-proliferative activity, indicating that the 28-O-glycoside moiety and methoxycinnamoyl group were essential for the HUVEC-selective growth inhibition of senegasaponins. Senegin III (2) inhibited the vascular endothelial growth factor (VEGF)-induced in vitro tubular formation of HUVECs and basic fibroblast growth factor (bFGF)-induced in vivo neovascularization in the mouse Matrigel plug assay. Moreover, senegin III (2) suppressed tumor growth in the ddY mice s.c.-inoculated murine sarcoma S180 cells. The analysis of the action mechanism of senegin III (2) suggested that the induction of pigment epithelium-derived factor (PEDF) would contribute to the anti-angiogenic effects of senegasaponins.

Keywords

Senegasaponins Polygala senega Angiogenesis Cancer HUVECs Tubular formation

Copyright information

© The Japanese Society of Pharmacognosy and Springer 2010