Journal of Soils and Sediments

, Volume 13, Issue 9, pp 1611–1625

Integrated toxicity evaluation of a pulp deposit using organisms of different trophic levels

  • Cornelia Kienle
  • Miriam Langer-Jaesrich
  • Daniela Baumberger
  • Doris Hohmann
  • Sergio Santiago
  • Heinz-R. Köhler
  • Daniel Zürrer
  • Almut Gerhardt
SEDIMENTS, SEC 1 • SEDIMENT QUALITY AND IMPACT ASSESSMENT • RESEARCH ARTICLE

DOI: 10.1007/s11368-013-0733-z

Cite this article as:
Kienle, C., Langer-Jaesrich, M., Baumberger, D. et al. J Soils Sediments (2013) 13: 1611. doi:10.1007/s11368-013-0733-z

Abstract

Purpose

In order to assess possible adverse effects originating from pulp deposits in a Swiss lake, a sediment quality triad approach was applied with chemical, ecotoxicological and ecological assessment methods.

Materials and methods

To obtain an integrative picture of the potential ecotoxicological effects on organisms of different trophic levels, four test procedures were applied. The acute effects of pulp deposit pore water on a decomposer, the amphipod Gammarus fossarum, were monitored. Chronic toxicity of the pore water was evaluated on primary producers via a growth inhibition test with unicellular green algae (Pseudokirchneriella subcapitata) and on secondary consumers in a reproduction test with the water flea Ceriodaphnia dubia. To evaluate the effects of the pulp deposit on sediment inhabitants, a whole-life-cycle test with the non-biting midge Chironomus riparius was undertaken. Chemical assessment included dissolved organic carbon, extractable organic halogenic compounds, polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs) and heavy metals. The composition of the macrozoobenthos community was analysed in order to assess the ecological effects.

Results and discussion

G. fossarum displayed increased locomotor activity at 12.5% but not at 25% sample concentration during a short-time exposure of 20 h. Chronic effects compromised the reproduction and growth of C. dubia (lowest observed effect concentration, 12.5% sample concentration) with zero population growth in 100% pulp deposit pore water. In 100% pulp deposit, C. riparius exhibited increased mortality at 10 and 17 days after oviposition. Pulp deposits of 50% and 100% concentration caused a significantly lower emergence compared with the reference treatments (lake sediment and quartz sand). Additionally, the locomotor activity of chironomids decreased significantly in 25–100% pulp deposit. No chronic effects of pulp deposit pore water on algae photosynthesis and growth could be detected. The bioassay results were in accordance with an elevated content of PAHs, PCBs and metals in the pulp deposit. Significantly more organisms known to be tolerant to organic pollution were present within the macrozoobenthos community.

Conclusions

In general, for sediment inhabitants such as chironomids, the pulp deposit has to be classified toxic. In the present test setup, the toxicity of the pulp deposit was reflected better by the chronic test systems applied than by the acute ones. The applied testing framework could be a suitable tool to assess the risk of contaminated sites, and this information will help decide whether risk mitigation measures should be taken. In addition, with a similar approach, the success of any mitigation measures taken can be assessed.

Keywords

Integrated assessmentSediment quality assessmentSediment triadTrophic levels

Supplementary material

11368_2013_733_MOESM1_ESM.docx (31 kb)
Online Resource 1Data for 2 h photosynthesis and 24 h growth inhibition of Pseudokirchneriella subcapitata [%] exposed to different dilutions of the lake water, lake reference sediment pore water and pulp deposit pore water. On the right side of the table the values for the blank are given (n = 2 replicates) (DOCX 30.6 KB)
11368_2013_733_MOESM2_ESM.pdf (107 kb)
Online Resource 2Data for locomotor and ventilatory activity [%] of G. fossarum after 2 and 20 h exposure to different dilutions of lake reference sediment pore water and pulp deposit pore water (n = 5 individuals per treatment) (PDF 107 kb)
11368_2013_733_MOESM3_ESM.pdf (166 kb)
Online Resource 3Data for population growth of C. dubia after 8 days exposure to three different samples and sample dilutions (shown in % relative to control). n = 11–14 replicates per treatment each with one adult C. dubia) (PDF 166 kb)
11368_2013_733_MOESM4_ESM.pdf (96 kb)
Online Resource 4Data for average survival [%] of C. riparius exposed to different sediments and sediment dilutions 10 and 17 days after oviposition. n = 4 replicates per treatment each with 33 larvae, lake reference sediment with n = 8 replicates (PDF 96 kb)
11368_2013_733_MOESM5_ESM.pdf (106 kb)
Online Resource 5Data for locomotor and ventilatory activity [%] of C. riparius 17 days after oviposition exposed to three different sediments and sediment dilutions during 2 h of behaviour measurement. n = 11 larvae per treatment, lake reference sediment with n = 28 larvae) (PDF 106 kb)
11368_2013_733_MOESM6_ESM.docx (31 kb)
Online Resource 6Data for mean cumulative number of emerged Chironomus riparius imagos [%] exposed to different sediments and sediment dilutions. n = 4 replicates per treatment each with 33 larvae, lake reference sediment with n = 8 replicates) (DOCX 31.4 KB)
11368_2013_733_MOESM7_ESM.docx (29 kb)
Online Resource 7Emerged Chironomus riparius imagos [%] exposed to different sediments and sediment dilutions at test end (day 33) (n = 4 replicates per treatment each with 33 larvae, lake reference sediment with n = 8 replicates) (DOCX 28.6 KB)
11368_2013_733_MOESM8_ESM.pdf (209 kb)
Online Resource 8Number of benthos organisms for different taxonomic groups per kilogram of sediment (dry weight) in the lake reference sediment (a) and the pulp deposit (b) determined in eight replicates each and extrapolated to 1 kg of sediment (PDF 209 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Cornelia Kienle
    • 1
  • Miriam Langer-Jaesrich
    • 2
  • Daniela Baumberger
    • 1
  • Doris Hohmann
    • 3
  • Sergio Santiago
    • 4
  • Heinz-R. Köhler
    • 2
  • Daniel Zürrer
    • 5
    • 6
  • Almut Gerhardt
    • 1
  1. 1.Swiss Centre for Applied Ecotoxicology Eawag/EPFLDübendorfSwitzerland
  2. 2.Animal Physiological EcologyUniversity of TübingenTübingenGermany
  3. 3.Eawag Aquatic Research, Department of Aquatic EcologyKastanienbaumSwitzerland
  4. 4.Soluval SantiagoCouvetSwitzerland
  5. 5.CSD Engineers and Geologists LtdZürichSwitzerland
  6. 6.Baudirektion Kanton ZürichZurichSwitzerland
  7. 7.LimCo International, Technologiezentrum KonstanzKonstanzGermany