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Response of extracellular carboxylic and thiol ligands (oxalate, thiol compounds) to Pb2+ stress in Phanerochaete chrysosporium

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Abstract

When exposed to higher Pb2+ concentration, Phanerochaete chrysosporium secreted higher content of oxalate and thiol compounds. An earlier and faster increase in oxalate was observed after short-term exposure, comparing with a gentle increase in the thiol compounds. In the extracellular polymeric substances (EPS) extract, more oxalate and T-SH were detected when the initial Pb2+ was higher, and the variations were different from the situation in the culture medium. In EPS solution, the oxalate amount was more closely related with Pb than that of thiol compounds. pH condition in the whole Pb removal process by P. chrysosporium ranged from 4 to 6.5 and was more beneficial for the binding of Pb2+ to carboxylic groups in the oxalic acid. More Pb2+ induced more EPS amount, and the increase of EPS amount influenced the immobilized oxalate more seriously. The present study can supply more comprehensive information about the metal passivation mechanism in white-rot fungi and provide meaningful references for an enhanced removal of heavy metals.

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Acknowledgments

The study was financially supported by the National Natural Science Foundation of China (51278176, 51378190, and 51408206), the Environmental Protection Technology Research Program of Hunan (2007185), the New Century Excellent Talents in University (NECT-13-0186), the Young Teacher Growth Program of Hunan University, the Scientific Research Fund of Hunan Provincial Education Department (521293050), the Fundamental Research Funds for the Central Universities, the Hunan University Fund for Multidisciplinary Developing (531107040762), the Hunan Provincial Innovation Foundation for Postgraduate (CX2014B141), and the Program for Changjiang Scholars and Innovative Research Team in University (IRT-13R17).

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Correspondence to Guangming Zeng or Danlian Huang.

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Responsible editor: Philippe Garrigues

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Li, N., Zeng, G., Huang, D. et al. Response of extracellular carboxylic and thiol ligands (oxalate, thiol compounds) to Pb2+ stress in Phanerochaete chrysosporium . Environ Sci Pollut Res 22, 12655–12663 (2015). https://doi.org/10.1007/s11356-015-4429-3

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